Volume 3 nr 1 / 2011 - Academia Oamenilor de Stiinta din Romania

Ion V. Popescu, Cristiana Radulescu, Claudia Stihi,38 Gabriela Busuioc, Anca Irina Gheboianu, Valerica Gh. CimpocaThe elements Zn, Cu, Fe, K, Mn, Mg, P, Se, Cd, Cr, Ni, Pb, Ti, Sr, Co and Biwere determined by Energy Dispersive X-Ray Fluorescence (EDXRF)Spectrometry and Atomic Absorption (AA) Spectrometry.From the same collecting point were taken n = 6 samples from the young fruitingbodies of wild young mushrooms species and their substrate at different times ofthe day: morning, afternoon and mid-day. The pH between 4.5 and 6.2 of forestsites of studied mushrooms species have been determined according to ISO10390:2005.2. Materials and Methods2.1. MaterialsThe young mushrooms species, Amanita vaginata, Amanita rubescens, Amanitaphalloides, Armillariella mellea, Armillariella tabescens, Agaricus campestris,Hypholoma fasciculare, Hypholoma pudorinus (Table 1) were collected from tenforest sites of Dambovița county, Romania, in the same direction of wind.Usually, the mushrooms represent the fruiting body (carpophore, mycocarp),mostly above ground, of higher fungi.Collections of species were made at different times of the day: morning, afternoonand mid-day by uprooting its substratum with aid of the scalpel.A fruiting body of mushroom species is formed from spacious undergroundmycelia (hyphae) by the process of fructification.Mycelia of ectomycorrhizal species live in symbiosis with roots of a plant, mostlya tree.The fruiting body samples have been washed with deionised water, from dirt,then, with a plastic knife, have been chopped up in 1 mm portions; the sampleshave been dried at 60 between 10 and 24 hours (depends of the species), thengrinded until to fine powder and finally weighed (CEN Standard ‘Foodstuffs —Determination of trace elements —Performance criteria, general considerationsand sample preparation).Substrate and soil samples have been dried at 70 0 C in 24 hours. After drying thesolid samples have been grinded until to fine powder and weighed. Chemicalsused included nitric acid (65% Aldrich), hydrochloric acid (37% Fluka), hydrogenperoxide (30% Fluka), and potassium chloride (Aldrich). Distilled deionised waterhad a resistivity better than 17.5 MΩ cm.The solutions used for calibration of FAAS were prepared from standard solution(Merck) of elements studied.Copyright © Editura Academiei Oamenilor de Știință din România, 2011Watermark Protected