Annual Report - IHBT

CSIR-IHBT Annual Report 2010-11Exploratory studies on climate change and adaptation of species complexes(NWP-0020)The physical, soil and geological maps of the study areas (Dhauladhar Wildlife Sanctuary, GreatHimalayan National Park, Rupi-Bhabha Wildlife Sanctuary and Pin Valley National Park) wereprepared in Geographic Information System (GIS) environment. These maps were prepared byscanning, geo-referencing and digitization of the hard copy maps published by Survey of India(SOI), Dehradun; National Bureau of Soil Survey & Landuse Planning (NBSS & LUP), Nagpur;and Wadia Institute of Himalayan Geology, Dehradun, respectively. The physical map depictsinformation on major locations, transport network, contours, glaciers and rivers in the study area.The soil and geological maps provide information on soil taxonomy and lithological units of theregion, respectively.In seed biology studies, seeds of Rumex nepalensis collected from different altitudes differed in theirgermination response such that the percent germination in high altitude ecotypes showed greaterdecline with rise in temperature. However, the optimum seed germination in Trifolium repensoccurred at 20°C.The transcriptome of P. kurrooa was analysed at high (HPSC) and low picrosides synthesizing(LPSC) conditions using next generation sequencing platform. HPSC and LPSC representeddifferent temperature regimes expected during climate change scenario. For HPSC, a total of 31338assembled transcripts were obtained with 100bp as the minimum length, 403.87bp as average lengthat 64.68 average coverage. A total of 2029 assembled transcripts (6.48%) were longer than 1000bp,while the longest assembled transcript was 5326bp. For LPSC, 63718 assembled transcript sequenceswere obtained with minimum 100bp length, 434.39bp average length and an average coverage of71.26. Sequences longer than 1000bp were 4988 with a maximum sequence length of 5210bp.Data showed up-regulation of the pathway genes at HPSC.7