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Annual Report - IHBT

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CSIR-<strong>IHBT</strong> <strong>Annual</strong> <strong>Report</strong> 2010-11PLANTATION CROPSTEA (Camellia sinensis)Construction of integrated linkage framework mapIn continuation to previous studies, the existing pseudo test cross progeny of SA-6 (R)xAsha(S) comprising of 212 individuals were selected for establishing the marker trait association forblister blight resistance (Fig. 39). A total of 572 primers were tested for identification of markersdistinguishing the parental lines and respective bulks. Molecular analyses identified 1619 markersdistinguishing the parental lines, whereas only 197 were able to identify the respective bulks.So far, 190 markers (AFLP, RAPD, SSR) were utilized for genotyping of 212 F 1individuals.Complete genotyping identified 260 markers in 1:1 ratio segregation.Fig. 39 Representative profile of F 1individuals of pseudo-test cross progeny of selected parental groups SA-6(R)xAsha (S) genotyped with primer combination TEGMS 65.41

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