Annual Report - IHBT

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PLANT PROTECTIONDiscovery and development of pest management agents from herbal sources(NWP037)Plant based pesticides are potential alternative to chemical pesticides for sustainable managementof insect pests. In evaluation of 331 plant extracts/fractions against Aphis craccivora, Plutellaxylostella, Spodoptera litura, Helicoverpa armigera and Tetranychus urticae, CSM 2169 P02 A001 againstdiamondback moth and CSM 2153 P02 A001 & RJO 2681 P03 A001 against aphids were foundto be effective. In the process of developing a commercial product, 8kg extract of RJO-2355 wasprepared by NEIST, Jorhat. Based on toxicological data, the botanical dust formulation NCL49was identified to be risk free.Larvicidal potential of essential oil/fractions and structure-activity relationshipstudiesEssential oil and fractions of Himalayan cedar (Cedrus deodara) were evaluated for insecticidalactivities against 2 nd instar of diamondback moth (P. xylostella). Solvent extraction by pentane wasidentified to be the most toxic (LC 50=287μg/ml) fraction followed by himachalene enrichedfraction (LC 50=362μg/ml).In another study, a series of chalcones (A–CH=CH–CO–B) synthesized under microwaveirradiation showed pesticidal activity against P. xylostella. The compound with para-Cl substitutionon rings A and B showed maximum activity (LC 50=170.24µg/ml).Ageratum enation virus (AEV) infecting common weedIn continuation to earlier studies, the commonly occurring weeds viz. Crassocephalum crepidioidesand Ageratum conyzoides were found infected with begomoviruses in the Palampur region of H.P.Complete geminiviral components of approximately 2.8 and 1.3 kb from the weeds showedassociation of AEV and a nanovirus-like DNA1 with C. crepidioides and A. conyzoides. Theirsequences were submitted to GenBank database under the accession numbers FN794201 andFN794202, respectively. In phylogenetic analyses, all Palampur isolates formed a separate cladewhile rest of the isolates grouped separately showing their geographical relatedness (Fig. 57).Chilli leaf curl Palampur virusCapsicum frutescens (chilli) were found associated with a begomovirus and a betasatellite likemolecule. The begomoviral genome (FM877858) and the betasatellite (FM877803) consisted of2775 and 1376 nucleotides (nt), respectively and appeared to be monopartite. In phylogeneticanalysis, the sequence grouped with an isolate of Papaya leaf curl virus-[Pakistan:2010] (FM955601)reported from Rhynchosia capitata and Tomato leaf curl New Delhi virus-[India:2006] (DQ629102)reported from tomato. The betasatellite showed maximum identity (94%) with chilli leaf curlbetasatellite (AM279671) from Pakistan (Fig. 58).63
CSIR-IHBT Annual Report 2010-11Fig. 57 Vein yellowing symptoms in a) C. crepidioides, b) A. conyzoides and c) Phylogenetic analysis of AEV fromvarious weeds. IN: India; PK: Pakistan; NE: NepalFig. 58 Phylogenetic trees of a) genomic component and b) betasatellite of begomoviruses64
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© DIRECTOR, CSIR-IHBT, PALAMPURPub
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CONTENTSFrom the Director’s deski
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CSIR-IHBT Annual Report 2010-11Plan
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CSIR-IHBT Annual Report 2010-11Fig.
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CSIR-IHBT Annual Report 2010-11Init
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CSIR-IHBT Annual Report 2010-11of t
Page 21 and 22: GENOMICS AND PROTEOMICSPathway engi
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Page 25 and 26: BIOINFORMATICSComputational biology
Page 27 and 28: NANOBIOLOGYSyzygium cumini and Bauh
Page 29 and 30: NATURAL PRODUCTS CHEMISTRYNATURAL C
Page 31 and 32: CSIR-IHBT Annual Report 2010-11Tabl
Page 33 and 34: CSIR-IHBT Annual Report 2010-11Oxid
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Page 37 and 38: CSIR-IHBT Annual Report 2010-11Chem
Page 39 and 40: CSIR-IHBT Annual Report 2010-11Fig.
Page 41 and 42: CSIR-IHBT Annual Report 2010-11Two
Page 45 and 46: CSIR-IHBT Annual Report 2010-11CAPI
Page 47 and 48: CSIR-IHBT Annual Report 2010-11TIRM
Page 51 and 52: CSIR-IHBT Annual Report 2010-11Sele
Page 53 and 54: CSIR-IHBT Annual Report 2010-11Mole
Page 55 and 56: CSIR-IHBT Annual Report 2010-11Head
Page 59 and 60: EDIBLE and SPICE CROPSAPPLE (Malus
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Page 63 and 64: CSIR-IHBT Annual Report 2010-11In c
Page 65 and 66: CSIR-IHBT Annual Report 2010-11Maxi
Page 71: CSIR-IHBT Annual Report 2010-11Enzy
Page 75 and 76: CSIR-IHBT Annual Report 2010-11Bact
Page 77 and 78: PROJECTS FUNDED BY CSIR UNDER EMPOW
Page 80 and 81: CSIR-IHBT Annual Report 2010-11CSIR
Page 82 and 83: CSIR-IHBT Annual Report 2010-11Them
Page 84 and 85: CSIR-IHBT Annual Report 2010-11FLOR
Page 86 and 87: CSIR-IHBT Annual Report 2010-11Trai
Page 88 and 89: CSIR-IHBT Annual Report 2010-11Prom
Page 90 and 91: CSIR-IHBT Annual Report 2010-11(Dal
Page 92 and 93: CSIR-IHBT Annual Report 2010-11Cult
Page 94 and 95: CSIR-IHBT Annual Report 2010-11Plan
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Page 100 and 101: SUPPORT SERVICESEngineering Service
Page 102 and 103: CSIR-IHBT Annual Report 2010-11IHBT
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Page 110 and 111: CSIR-IHBT Annual Report 2010-11Sanj
Page 112 and 113: CSIR-IHBT Annual Report 2010-11Jait
Page 114 and 115: CSIR-IHBT Annual Report 2010-11Mahi
Page 116 and 117: CSIR-IHBT Annual Report 2010-11Shar
Page 118 and 119: CSIR-IHBT Annual Report 2010-11Sood
Page 120 and 121: CSIR-IHBT Annual Report 2010-11CONF
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CSIR-IHBT Annual Report 2010-11Kuma
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CSIR-IHBT Annual Report 2010-11Uniy
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CSIR-IHBT Annual Report 2010-11AWAR
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CSIR-IHBT Annual Report 2010-11Arvi
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CSIR-IHBT Annual Report 2010-11MK S
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CSIR-IHBT Annual Report 2010-11GUES
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CSIR-IHBT Annual Report 2010-11Work
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CSIR-IHBT Annual Report 2010-11•
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CSIR-IHBT Annual Report 2010-11IMPO
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CSIR-IHBT Annual Report 2010-11fgUn
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CSIR-IHBT Annual Report 2010-11Inau
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CSIR-IHBT Annual Report 2010-11Fern
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CSIR-IHBT Annual Report 2010-11IHBT
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CSIR-IHBT Annual Report 2010-11Moti
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CHAIRMANProf. Sudhir K Sopory, FNAV
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DirectorDr. PS AhujaScientist Gr. I
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CSIR-IHBT Annual Report 2010-11NEWL
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