Annual Report - IHBT

ELSHOLTZIA (Elsholtzia fruiticosa)Thirty-five constituents were identified in Elsholtzia oil extracted by SCE and HD methods, whereas, 14volatile constituents were identified in head space (HS) analysis. The oil extracted by HS, HD and SCE werepredominated by non-terpenes (59.8%), oxygenated monoterpenes (41.1%) and sesquiterpene hydrocarbons(21.8%). Monoterpene hydrocarbons were determined to the extent of 13.6, 19.4 and 4.3% in HS, HD andSCE extractions (Table 9).Table 9 Variability of terpenes of E. fruiticosa essential oil with different extraction techniquesTerpenes SFE (%) HD (%) HS (%)Monoterpene hydrocarbons 4.3 19.4 13.6Oxygenated-monoterpenes 37.1 41.1 22.4Sesquiterpenes-hydrocarbons 21.8 6.6 1.1Oxygenated- sesquiterpenes 3.0 0.8 -Diterpenes 3.4 - -Non-terpenes 24.5 29.8 59.8Total 94.2 97.7 96.9EUCALYPTUS (Eucalyptus cinerea)Eucalyptus oil was extracted by hydrodistillation (HD) and supercritical carbon dioxide extraction (SCE)techniques. HD yielded highest oil (free volatiles) content (3.1%) in comparison to SCE (1.1%). Both thefree and bound volatiles contained 85.1 and 20.6% 1,8-cineole, respectively. Similarly, the oil produced bySCE was dominated by 1,8-cineole (70.4%) followed by oxygenated sesquiterpenes (6.4%) and sesquiterpenehydrocarbons (3.3%).