Abstracts (complete list) - Wissenschaft Online

Katja Kotsch, Vera Merck, Kristina Kunert, Anja Reutzel-Selke, Andreas Pascher, Hans-
Dieter Volk, Peter Neuhaus, Johann Pratschke
Identification of molecular candidate marker in zero kidney
biopsies are indicative for graft quality
In renal transplantation, allograft biopsies provide valuable diagnostic information.
Consequently, several attempts have been made to predict the early graft outcome by
histological and molecular analyses in zero-hour graft biopsies illustrating that subtle
inflammation and immune activation detected in the intraoperative period are
associated with adverse allograft outcome post transplantation. The clinical outcome is
dependent upon various risk factors including donor brain death or prolonged cold
ischemia (CI). However, current knowledge about the influence of brain death and CI is
only restricted to a low number of genes and the molecular and cellular mechanisms are
thus far unknown. Efficient strategies to prevent prolonged cold ischemia or brain death
related complications require a better understanding of the molecular processes
reflecting intragraft inflammation present intraoperatively at the zero-hour. We
therefore studied mRNA gene expression of zero kidney biopsies derived from 63
cadaveric donors (CAD) (CI=13,3 ± 3,6) and 27 living donors (LD) (CI=2,8 ± 0,7).
Intraoperative biopsies were taken 30 min. after vascular reperfusion and were
immediately snap-frozen. Messenger RNA was extracted and gene expression was
measured by real-time RT-PCR. Recently it has been demonstrated that the induction of
the T cell marker CD3 or the intercellular adhesion molecule (ICAM)-1 in zero biopsies
are predictive of acute rejection (Avihingsanon et al. 2005). By comparing zero biopsies
from CAD versus LD we observed a significant induction of CD3 (p