Abstracts (complete list) - Wissenschaft Online

Heribert Appelhans, Michael Walther, Andrea Friße, Michael A. Harvey, Stephen A.
Judice, Brett A. Stillman, Hans Peter Seelig
A CE-Certified Autoimmune Disease Diagnostic Protein
Microarray
CombiChip® Autoimmune is an auto-antigen microarray that enables laboratories to
measure the presence of 14 different auto-antibodies associated with collagenosis and
vasculitis-related autoimmune diseases simultaneously from a single serum sample.
Thirteen protein antigens, double stranded DNA, and an internal calibrator (human IgG)
are arrayed on each of 16 pads of nitrocellulose on a coated glass microscope slide
(FAST® Slide). Seventy microliters of diluted patient’s serum is added to each array,
and the presence of reactive antibodies is detected with DY647-conjugated anti-human
IgG. The calibrator, a series of dilutions of human IgG arrayed on each pad, is used to
construct a standard curve to quantify the levels of autoimmune antibodies present
against each antigen. This microspot immunoassay yields reproducible results which are
in good agreement with standard methods such as ELISA and Western Blot. It greatly
reduces assay time and delivers an auto-antibody profile for each patient. This
diagnostic assay has been validated to ensure compliance with the CE guidelines for invitro
diagnostics.