Abstracts (complete list) - Wissenschaft Online

Matthias Bros, Frank Jährling, Andrea Renzing, Nadine Wiechmann, Ngoc-Anh Dang,
Arne Sutter, Ralf Ross, Jürgen Knop, Stephan Sudowe, Angelika B. Reske-Kunz
A newly established murine immature dendritic cell line
exerts tolerogenic function at its immature state and upon
alternative activation in the presence of glucocorticoid
The phenotype and function of murine dendritic cells (DC) is primarily studied using
bone-marrow-derived dendritic cells (BM-DC), but may be hampered by the
heterogenous phenotype of BM-DC due to differential state of maturation. We
characterized a newly established murine DC line (SP37A3) of myeloid origin. During
maintainance in the presence of GM-CSF and M-CSF, SP37A3 cells resemble immature
DC characterized by low expression of MHC II and costimulatory molecules and low T
cell stimulatory capacity. Upon stimulation, SP37A3 cells acquire a mature phenotype
and activate naive T cells as potently as BM-DC. SP37A3 cells at their immature state as
well as upon alternative activation by TNF-a and IL-1ß in the presence of
dexamethasone (DEX) induced regulatory T cells which were anergic upon restimulation
and suppressed proliferation of naive T cells. Alternatively activated SP37A3 cells
displayed lower expression levels of costimulatory molecules and proinflammatory
cytokines as compared with mature cells, as well as upregulated expression of FcgRIIB
and IL-1RA. SP37A3 cells were responsive to DEX even when applied at later time
points during activation suggesting functional plasticity. Taken together, the myeloid DC
line SP37A3 represents a suitable model to study functions of myeloid dendritic cells at
tolerogenic and immunogenic state, circumventing restrictions associated with the use
of primary DC and BM-DC.