Abstracts (complete list) - Wissenschaft Online

Uwe Kolsch, Christina Weber, Caroline Ritter, Anna Viehbahn, Thomas Brune
THE REPRODUCTIVITY OF MICE IS INDEPENDENT OF A MHC-I
HAPLOTYPE MISMATCH BETWEEN THE MOTHER AND THE
FETUS
Introduction: Recent investigations have shown fetal survival depending upon
maternal TH2-switch and existence of regulatory T-cells. Up to now it is unclear if fetal
survival depends on maternal TH1/TH2 basal immune responses and on MHC-recognition
and MHC-haplotyp, factors influencing MHC - T cell interaction. Methods: To address
the question whether different types of basal immune responses influence
reproductivity, inbred mouse strains with different TH1/TH2-reactivities and different
MHC haplotypes (CBA, Balb/C, C57BL/6, DBA1) were crossed to F3 generation. TH1/TH2 imbalances were measured by CD69-upregulation in mixed lymphocyte cultures (MLC)
from parental animals. Results: CD8+-lymphocytes from TH1 reacting strains are
activated to a higher extent when compared to lymphocytes of TH2 reacting strains. In
contrast immune responses between MHC I-haplotype homozygous F2 and F3-animals
are balanced. Numbers of progeny were significantly reduced when male TH2-reacting mice were crossed with female TH1-reacting mice compared to breedings from female
TH2-reacting mice crossed with male TH1-reacting mice. The latter was comparable to
F1-hybrid breeding pairs and breeding pairs of MHC I-haplotype homozygous F2 mice.
There was no difference in progeny numbers when MHC I-haplotype homozygous F2
mice with total or no MHC mismatch were crossed. Discussion: TH2 reactivity is
advantageous for pregnancy outcome but TH2 switching is independent of MHC Ihaplotype.
Breeding of genetically non identical mice positively influences pregnancy
outcome independently of the MHC I-haplotype.