Abstracts (complete list) - Wissenschaft Online

Jessica Butz, Cordula Fuchs, Barbara Kessler, Heiner Voigt, Daniel Wienhold, Mathias
Buettner
Immune reaction of swine after repeated intra-muscular (i.
m.) immunization with avian influenza virus H5
In swine there is little evidence of virus host inaction during the recent avian influenza
virus (AIV) H5N1 epidemics in Asia and South East Asia. To induce and characterize an
immune response in swine inactivated H5N1 (mallard/Bavaria/1/06)) was injected two
times intra-muscular (i. m.) followed by a third i. m. injection of live low pathogenic
H5N2 AIV subtype.
In total ten animals were immunized, five without and five with parapoxvirus as an
adjuvant.
Only after the second and third application H5-specific antibodies were detected in
swine sera with slightly higher titers in the sera of the adjuvant immunized animals. In
vitro re-stimulation of peripheral blood mononuclear cells (PBMC) with various live AIV
subtypes including the vaccine virus and swine pathogenic subtypes did not result in
significant antigen mediated interferon gamma secretion as measured by ELISPOT. The
PBMC from immunized animals also showed no or weak interferon alpha secretion after
AIV stimulation in contrast to other control viral stimuli. Possible immune response
counteracting activity of virulent avian influenza A subtypes will be investigated.