Abstracts (complete list) - Wissenschaft Online

Sandra Martina Dittrich, Elfriede Noessner, Hans Demmelmair, Gernot Desoye, Dolores
Schendel, Berthold Koletzko, Susanne Krauss-Etschmann
Influence of n3/n6 polyunsaturated fatty acids on placental
immune responses
Introduction
N3/n6-long chain polyunsaturated fatty acids (LC-PUFA) modulate immune responses.
In addition, docosahexaenoic acid (n3, DHA) and arachidonic acid (n6, AA) are
important for the neural development and selectively transported to the fetus. However,
it is unknown how n3/n6-LC-PUFA affect placental immune responses.
Material and methods
Basal mRNA expression levels of IL-6, IL-8, IL-12, TGF-β, MCP-1 and RANTES were
quantified in choriocarcinoma lines (ACH-3P, BeWo, JAR, JEG) and eight non-placental
carcinoma lines by real time RT-PCR (iCycler, Fa. Biorad). Selected cell lines were
incubated with 1•M, 10•M and 100•M DHA, eicosapentaenoic acid (EPA) and AA using
monounsaturated oleic acid (OA) and saturated palmitic acid (PA) as controls. Cytokine
expression patterns and the cell proliferation rate (cellscreen, Fa. Innovatis) were
determined after 1h, 6h, 24h and 48h.
Results
Basal mRNA expression levels of IL-6, IL-8, TGF-β, MCP-1 and RANTES were
comparable in JAR, ACH-3P, RCC 26 (renal cell carcinoma) and MCF-7 (mamma
carcinoma). IL-12 mRNA was detectable only in BeWo, Du 145 (prostate carcinoma)
and HEK 293 (human, adenovirally immortalised embryonic kidney cell line). PA
increased the proliferation rate of JAR and MCF-7. EPA increased the proliferation rate of
JAR. PA and OA slightly increased IL-6 and RANTES mRNA expression levels, while EPA
decreased IL-6 mRNA expression levels in JAR. There was no effect of any fatty acid on
RCC 26.
Conclusion
Choriocarcinoma lines had heterogeneous cytokine expression patterns. Even very
related choricarcinoma lines showed clear differences. EPA, PA and OA modulated
proliferation rates and cytokine expression levels. However, the changes were small and
need further confirmation.