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RF <strong>AB</strong> JBT JOURNAL OF BIOMOLECULAR TECHNIQUES Clive A. Slaughter St. Jude Children’s Research Hospital 332 North Lauderdale Street Memphis, TN 38105-2794 Tel: (901) 495-4844 Fax: (901) 495-2945 email: clive.slaughter@stjude.org Ruth Angeletti Albert Einstein College of Medicine Kalyan R. Anumula SmithKline Beecham Pharmaceutical Lynda Bonewald University of Texas Health <strong>Science</strong> Center at San Antonio Gerald Carlson University of Missouri Steven Carr SmithKline Beecham Pharmaceutical Dan Davison Bristol-Myers Squibb EDITOR-IN-CHIEF Clayton W. Naeve St. Jude Children’s Research Hospital 332 North Lauderdale Street Memphis, TN 38105-2794 Tel: (901) 495-3861 Fax: (901) 495-2945 email: clayton.naeve@stjude.org ASSOCIATE EDITORS EDITORIAL BOARD Karen De Jongh Zymogenetics Greg Grant Washington University at St. Louis George Grills Albert Einstein College of Medicine Susan Hardin University of Houston Preston Hensley Pfizer, Inc. Harold Hills Iowa State University Paul Morrison Dana-Farber Cancer Institute COPPERPLATE PRESS, INC. 85 East Stewart Avenue Lansdowne, Pennsylvania 19050 Tel: (610) 284-5257 Fax: (610) 284-5258 email: mconnors@copperplatepress.com http://www.copperplatepress.com David Speicher The Wistar Institute 3601 Spruce Street Philadelphia, PA 19104-4268 Tel: (215) 898-3972 Fax: (215) 898-0664 email: speicher@wistar.upenn.edu Ron Niece Research Resources and Technologies Margaret Robertson University of Utah Richard Simpson Ludwig Institute for Cancer Research Laurey Steinke University of Nebraska Medical Center John Stewart University of Colorado Dan Strydom BioNebraska, Inc. John R. Yates University of Washington ASSOCIATION OF BIOMOLECULAR RESOURCE FACILITIES
JBT JOURNAL OF BIOMOLECULAR TECHNIQUES VOLUME 11, ISSUE 4, DECEMBER 2000 METHODS & REVIEWS CONTENTS Implementation of Automation in a Small-Scale DNA Sequencing Core Facility 151 Jennifer L. Ware, Laurie Moran, Ching-Lun Lin, and Barton Slatko RESEARCH GROUP REPORTS Strategies for the Synthesis of Labeled Peptides 155 Lisa Bibbs, Nicholas P. Ambulos, Steven A. Kates, Ashok Khatri, Katalin F. Medzihradszky, George Ösapay, and Susan T. Weintraub REPORT Research Equipment and Resource Requirements of NIH-Supported Investigators: 166 An Assessment of Current Conditions and Recommendations for Future Funding and Programs Tamara R. Zemlo, Howard H. Garrison, David Lester, Mustafa Lokhandwala, Fred Naider, Stephen White, and David W. Speicher BOOK REVIEWS Amino Acid Analysis Protocols, edited by Catherine Cooper, 177 Nicolle Packer, and Keith Williams Reviewed by Alan Mahrenholz — continued — The Journal of Biomolecular Techniques (ISSN 1524-0215) is published quarterly by The Association of Biomolecular Resource Facilities, 1201 Don Diego Avenue, Santa Fe, NM 87505. Copyright © 2000 by The Association of Biomolecular Resource Facilities. All rights reserved. Periodicals postage paid at Bethesda, Maryland, and at additional mailing offices. Address for subscription information, orders, or changes of address: The Association of Biomolecular Resource Facilities, 1201 Don Diego Avenue, Santa Fe, NM 87505; Tel: (505) 983-8102; Fax: (505) 989-1073. Annual subscription rates: Members of the Association of Biomolecular Resource Facilities receive a subscription as part of their membership fee. Other subscribers are charged as follows. Within the United States: $75.00 individual, $100.00 institution. (All other countries add $21.00 for air freight shipping—delivery within 7 to 21 days, worldwide.) International subscriptions must be prepaid. Please add $4.00 to all rates above for handling. Single copies, when available, may be ordered from the publisher. Single copies: $25.00. Prices subject to change without notice. Copies will be replaced without charge if the publisher receives a request within 90 days of the mailing date, both U.S. and worldwide. POSTMASTER: Send address changes to The Association of Biomolecular Resource Facilities, 1201 Don Diego Avenue, Santa Fe, NM 87505.
Page 1: VOLUME 11, ISSUE 4, DECEMBER 2000 C
Page 5 and 6: CORPORATE SPONSORSHIP ABRF corporat
Page 7 and 8: Implementation of Automation in a S
Page 9 and 10: 0.8 pmol/�L with 5 �L per prime
Page 11 and 12: RESEARCH GROUP REPORTS Strategies f
Page 13 and 14: Matrix-Assisted Laser Desorption Io
Page 15 and 16: TABLE 1 Summary of Results from ABR
Page 17 and 18: FIGURE 4 removed using dilute TFA o
Page 19 and 20: FIGURE 8 FIGURE 9 Tandem mass spect
Page 21 and 22: REFERENCES 1. Angeletti RH, Bonewal
Page 23 and 24: shared research equipment. When ask
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Page 27 and 28: Survey Respondents’ Priorities fo
Page 29 and 30: FIGURE 9 FIGURE 10 Choices for gove
Page 31 and 32: FIGURE 11 Distribution of NIH Resea
Page 33 and 34: BOOK REVIEWS Amino Acid Analysis Pr
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Page 37 and 38: AMINO ACID COMPOSITION AND SEQUENCE
Page 39 and 40: emove contaminants. The bound prote
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Page 43 and 44: ABRF 2001 THE NEW BIOLOGY Technolog
Page 45 and 46: P5-M Using high speed data collecti
Page 47 and 48: P13-S Microsatellite analysis using
Page 49 and 50: P21-T Versalinx chemical affinity t
Page 51 and 52: P29-M Protein/DNA technology DSL ve
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P37-S Data-directed real-time instr
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P45-T Identification of changes in
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P53-M Nano-LC ion-trap mass spectro
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P61-S Genopure: a novel magnetic be
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P69-T Efficient detergent and Cooma
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P77-M The utilities of N-terminal s
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P85-S Rapid oligosaccharide mapping
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P93-T Analytical strategies for the
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P101-M Implementing surface plasmon
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P109-S Protein molecular communicat
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P117-T New separation medium for th
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P125-M A high sensitivity silver st
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P133-S Application of human cDNA mi
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P141-T Somatic embryony patterns an
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R3-M A current profile of microarra
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S4 A crack in the egg: protein-prot
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T1 Oligonucleotide synthesis chemis
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T9 More than one way to capture a p
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Key to Abstract Numbering Prefixes:
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Neitz, S., P38-M Nelson, J., P116-M
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Admon, Arie, 92 Bibbs, Lisa, 1 Bint
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JBT JOURNAL OF BIOMOLECULAR TECHNIQ
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