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NEWS & EVENTS field of biotechnology and as a mouthpiece for ABRF in the larger scientific community. He will be keenly missed by all associated with the Journal. Both Lynda Bonewald, retiring President of ABRF, and Mark Lively, incoming President, have expressed their gratitude to Clayton for services to the Society. The Publications Committee, chaired by Ralph Bradshaw, is seeking nominations from the membership of ABRF for a new Editor-in-Chief, and asks that these be sent in writing to Ralph at rablab@uci.edu. Dave Speicher will join Clive Slaughter as the newest Associate Editor for JBT. He and Clive will be reorganizing the review process and restructuring the editorial board. ABRF members can support JBT by submitting their best work to our society’s journal. Often, articles published in the field of biomolecular techniques present “results” but not detailed methods. ABRF members are encouraged to publish articles providing detailed techniques in JBT to be referenced in other articles. JBT will also publish Research Group reports targeted to ABRF members and including current and future technologies. ABRF Web Page Ted Thannhauser, chair of the Web Site Committee, has presented a plan to secure bids from commercial companies to update our Web Site. A list of desired features for the redesigned ABRF Web Page includes the following: the Web Site should serve as a window to other resources; Research Groups should be able to do online updates; there should be hyperlinks to corporate sponsors’ sites; and laboratories should be able to do online updates of their entries in the “yellow pages.” The option of banner advertising to generate revenue will be explored. New Board Members It is with regret that we bid farewell to John Stults and Karen DeJongh as they rotate off of the Executive Board as of January 1, 2001. John has provided insight and wisdom concerning ABRF operations in his usual quiet yet effective manner. Karen has been absolutely invaluable as treasurer of the organization and leaves this position with ABRF in good financial shape. Laurey Steinke will assume the Treasurer’s position, and Mark Lively will become President. We are looking 180 JOURNAL OF BIOMOLECULAR TECHNIQUES, VOLUME 11, ISSUE 4, DECEMBER 2000 forward to the participation of our newest board members, Ted Thannhauser and Preston Hensley. ABRF/ASBMB JOINT SYMPOSIUM The ABRF/American Society of Biochemistry and Molecular Biology (ASBMB) Joint Symposium will be presented at the 2001 ASBMB Annual and Satellite Meetings held in conjunction with Experimental Biology 2001, to be held March 31 through April 4 in Orlando, Florida. This is a continuing series sponsored by both ABRF and ASBMB. The goal of these joint symposia is to bring emerging technologies to biochemists and molecular biologists, who can then use the new techniques in their research programs. “High-Throughput Genomic Technologies: Decisions Based on Science and Reality” is scheduled for 9:45 A.M. on Wednesday, April 4. Michael R. Sussman, Director of University of Wisconsin Biotechnology Center, and Ronald L. Niece, Research Resources and Technologies, organized the symposium, which covers several different applications of developing technologies. The symposium includes an introduction highlighting the role of resource laboratories in contemporary research and illustrating how the instrumentation and experience of the core laboratory can expedite research programs. Dr. Sussman will discuss progress in the development of saturation reverse genetics using knockout plants and will describe a maskless array synthesizer for producing high-density DNA oligonucleotide arrays “on the fly.” Hundreds of thousands of lines can be screened for knockout plants for any gene of interest. Combinatorial chemistry comes to the laboratory benchtop in the form of high-density oligonucleotide arrays. Dr. Smith addresses the problem of complexity in mass spectral data. His laboratory’s approach is to reduce complex spectra by controlling the charge state of the ion to a small number. Using electrospray ionization and an orthogonal time-of-flight mass spectrometer, the aerosol is exposed to a bipolar ionizing gas. In mixtures of proteins or nucleic acids, spectra are simplified and chemical noise minimized.
AMINO ACID COMPOSITION AND SEQUENCE ANALYSIS Sechi S, Chait BT.A method to define the carboxy terminal of proteins. Anal Chem 2000;72:3374–3378. Proteins are first digested with lysylendopeptidease. Anydrotrypsin, a catalytically inert derivative of trypsin in which serine-195 is converted to dehydroalanine, is used to bind peptides containing a lysine (or arginine) residue at their C-termini. The peptide derived from the C-terminus of the intact protein, lacking a C-terminal lysine or arginine, is not retained by anhydrotrypsin and is collected for subsequent analysis. Matrix-assisted laser desorption/ionization (MALDI) is used to identify the peptide, and tandem mass spectrometry is used to sequence it. CARBOHYDRATES AND GLYCOPROTEINS Charlwood J, Skehel JM, Camilleri P.Analysis of N-linked oligosaccharides released from glycoproteins separated by two-dimensional gel electrophoresis. Anal Biochem 2000;284:49–59. Protocols are described for analysis of N-linked carbohydrates on glycoproteins that have been sub- ARTICLE WATCH This column highlights recently published articles that are of interest to the readership of this publication. We encourage ABRF members to forward information on articles they feel are important and useful to Clive A. Slaughter, St. Jude Children’s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105-2794; Tel: (901) 495-4844; Fax: (901) 495-2945; email: clive.slaughter@ stjude.org; or to any member of the editorial board. Article summaries reflect the reviewers’ opinions and not necessarily those of the Association. jected to two-dimensional gel electrophoresis. Glycans are released from the proteins by digestion with protein N-deglycosidase F (PNGase F), derivatized with 3-acetamido-6-aminoacridine, and analyzed by matrix-assisted laser desorption/ionization (MALDI)– time-of-flight mass spectrometry. Enzymic release of the glycan is demonstrated using in-gel digestion of the excised gel spot, with or without prior tryptic digestion, and also using glycoproteins electroblotted to polyvinylidene difluoride membranes. PHOSPHOLIPIDS Journal of Biomolecular Techniques 11:181–184 © 2000 ABRF RF AB Taguchi R, Hayakawa J,Takeguchi Y, Ishida M.Two-dimensional analysis of phospholipids by capillary liquid chromatography/electrospray ionization mass spectrometry. J Mass Spectrom 2000;35:953–966. A strategy for analysis of phospholipids extracted from cultured cells is described. The strategy uses capillary liquid chromatography combined with tandem mass spectrometry in a quadrupole mass analyzer. More than 500 species of phospholipids are included by the method. Positive molecular ions, negative molecular ions, positive fragment ions, and negative fragment ions are monitored, consecutively, in 11-second cycles. Positive molecular ions indicate choline-containing phospholipids such as phosphatidylcholine, sphingomyelin, lysophosphatidylcholine, and phosphatidylethanolamine. Negative JOURNAL OF BIOMOLECULAR TECHNIQUES, VOLUME 11, ISSUE 4, DECEMBER 2000 181
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Page 45 and 46: P5-M Using high speed data collecti
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Neitz, S., P38-M Nelson, J., P116-M
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Admon, Arie, 92 Bibbs, Lisa, 1 Bint
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