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P141-T<br />
Somatic embryony patterns and plant regeneration in cotton.<br />
B-H. Zhang; Cotton Res. Inst., Chinese Acad. of Agr. Sci., Baibi, Anyang,<br />
Henan 455112, People’s Republic of China<br />
Globular, heart, torpedo and cotyledon stages of development were observed<br />
in cotton somatic embryos, and only late torpedo and cotyledon stage somatic<br />
embryos could germinate into plantlets, the others could only produce<br />
advanentive embryos or adventive roots. Various developmental stage<br />
somatic embryos existed in cotton tissue cultue, and the number of the<br />
globular and heart-shaped stage was more than that of cotyledon embryos.<br />
The germination of cotton somatic embryos could be classified into three<br />
kinds and were affected by many factors such as hormons, basis medium et<br />
al. Resting phenomenon esists in cotton somatic embryos.<br />
P143-M<br />
Relationship between sexual maturation, serum leptin and<br />
sex hormone levels in obese and normal children.<br />
S.C. Hao, Y.C. Yuan; Harbin Med. Univ., China, 199 Dazhi Street,Harbin,<br />
Harbin, Heilongjiang 150001, People’s Republic of China<br />
In order to study the relationship between sexual maturation, serum leptin<br />
and sex hormone levels, the sexual maturation of 300 obese children and 300<br />
normal children not taking any medication or having evidence of endocrine<br />
or metabolic disease were questioned and their serum leptin and sex hormone<br />
levels were measured. Serum leptin levels were significantly increased<br />
(P � 0.01) in obese children compared with controls, while the sexual maturation<br />
of obese children was much earlier than that of controls (P � 0.01).<br />
Serum leptin levels were significantly increased (P � 0.01) in female compared<br />
with male, while the sexual maturation of female was much earlier<br />
than that of male (P � 0.01). There was significant negative correlation<br />
between testosterone and leptin in male (obese group: r � �0.83, P � 0.01;<br />
control group: r � 0.93, P � 0.01), while the estradiol was positive correlated<br />
with leptin in female (obese group: r � 0.84, P � 0.01; control group: r �<br />
0.95, P � 0.01). This study shows that leptin can promote sexual development<br />
in children and may be regulated by sex hormone. There are gender<br />
differences in the correlations between leptin and sex hormone, which may<br />
cause the differences of sexual maturation in male and female. This study<br />
also suggests that leptin play a more important role than sex hormones in the<br />
sexual development.<br />
POSTER <strong>AB</strong>STRACTS<br />
<strong>AB</strong>RF 2001 <strong>AB</strong>STRACTS<br />
P142-S<br />
The novel protein patterning method and its application<br />
for protein-protein interactions.<br />
B-G. Kim, C-S. Lee; Sch. of Chem. Engin., Seoul Natl. Univ., Seoul<br />
National University, Kwanak-Gu, Seoul, Seoul 151-742,<br />
Republic of Korea<br />
This research describes the new protein patterning method for the formation<br />
of highly defined two-dimensional protein arrays onto very hydrophobic thin<br />
film coated silicon substrate expressing protein functional activity at its patterned<br />
surface.<br />
In protein patterning process, Because very critical problem is non-specific<br />
protein binding onto undesirable region, We had tried a spin coated FluoroCarbon<br />
thin film, very hydrophobic surface property, which was expected<br />
to protect non-specific protein binding in our patterned silicon chip which<br />
was made from the lift-off process. From this simple method, we had obtained<br />
very high ordered protein patterns (180 �m, 300 �m, 500 �m diameter<br />
circle and 100 �m by 200 �m rectangular square) and also discarded<br />
nonspecific protein binding and blocking proteins which were generally<br />
used for protecting nonspecific binding such as bovine serum albumin(BSA)<br />
and skim milk proteins et al.<br />
Patterned micron-scale arrays were shown by fluorescence tagging proteins<br />
and had successfully obtained reliable quantitative data with confocal microscopy.<br />
And We also could create a versatile patterned protein surfaces for<br />
protein-protein interactions such as sandwich immuno-assay and indirect<br />
immuno-assay.<br />
From the these results, We have developed miniaturized assay systems showing<br />
the rapid, low sample volumes and high sensitivity.<br />
P144-T<br />
Experiences in implementing single nucleotide extension in a<br />
high throughput genotyping core facility.<br />
R. Scholl, L.W. Ballard; Univ. of Utah, 50 North Medical Dr. 4A430A,<br />
School of Medicine, Salt Lake City, UT 84132<br />
This poster will discuss some of our problems and successes in implementing<br />
single nucleotide extension (SNE) in a high throughput genotyping core<br />
facility as a method for DNA fragment analysis of SNP’s. We will discuss<br />
primer selection, PCR protocols, cleanup methods, quality control issues, and<br />
loading methods used. Product contamination and multiplexing of the samples<br />
will also be addressed.<br />
JOURNAL OF BIOMOLECULAR TECHNIQUES, VOLUME 11, ISSUE 4, DECEMBER 2000 223
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