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ARTICLE WATCH utility of the method, and the stabilization of maltosebinding protein on binding to maltose is also demonstrated. MICROARRAYS Lee MT, Kuo FC,Whitmore GA, Sklar J. Importance of replication in microarray gene expression studies: statistical methods and evidence from repetitive cDNA hybridizations. Proc Natl Acad Sci USA 2000;97:9834– 9839 This study investigates the inherent variability in gene expression data and assess the extent to which replication in an experiment produces more consistent and reliable findings. The results show that any single microarray output is subject to substantial variability, even though, by design, variability as a result of multiple preparations of probe, arrays on different slides, or arrays generated at different times is not admitted. A single output yields numerous misclassifications. Replications are not consistent and therefore produce different lists of expressed genes. Modeling the random variation in gene expression indicates that the probability that mRNA in the sample tissue 184 JOURNAL OF BIOMOLECULAR TECHNIQUES, VOLUME 11, ISSUE 4, DECEMBER 2000 either fails to be represented as probe or fails to hybridize to the cDNAs on the slide may be as large as 5% (false-negatives). The probability that ghost genes are expressed may be as large as 10% (falsepositives). When microarray data from several replicates are combined, however, a more accurate picture of gene expression is produced. It is recommended that at least three replicates be included in the design of experiments using cDNA microarrays. BIOINFORMATICS Fenyö D. Identifying the proteome: software tools. Curr Opin Biotechnol 2000;11:391–395. This article reviews the various software tools that are available on the internet for searching protein sequence databases using mass spectral information. Both peptide mapping data listing the mass values of proteolytic peptides and fragment ion data derived by collisional dissociation of peptides to yield sequenceinformative ions are included. Attention is paid to the methods used by the various tools to rank protein candidates and the methods by which the quality of identifications are assessed.
2001 DATES TO REMEMBER Jan 13–18 HPCE 2001, 14th International Symposium on Microscale Separations and Analysis, Boston, MA. Contact: Joan Oefner, Symposium Manager, Rhema Association Management, 156 South Spruce Avenue, Suite 207A, South San Francisco, CA 94080-4556; Tel: (650) 876-0792; Fax: (650) 876-0793 (email: joefner@casss.org). Jan 29–30 IBC’s Process Validation for Biologicals, San Diego, CA. Contact: Jim Prudhomme, Lifesciences Marketing Division, IBC USA Conferences, Inc., 1 Research Drive, Suite 400A, Southborough, MA 01581. Tel: (508) 616-5550 X205; Fax: (508) 616-5533 (email: jprudhomme@ibcusa.com; URL: http://www.ibcusa.com/2532). Feb 8–9 IBC’s 9th Annual Conference on Alzheimer’s, Atlanta, GA. Contact: Julie Anderson, Marketing Manager—Life Sciences, IBC USA Conferences, Inc., 1 Research Drive, Suite 400A, Westborough, MA 01581. Tel: (508) 616-5550 X423; Fax: (508) 616-5533 (email: janderson@ibcusa.com; URL: www.ibcusa.com/2579). UPCOMING EVENTS ABRF members and Corporate Sponsors are encouraged to make announcements about pertinent meetings, workshops, etc. that are beneficial to our members and open to the public. Send items to be listed to Daniel J. Strydom, BioNebraska, Inc., 3820 NW 46th St., Lincoln, NE 68524-1637 (Tel: 402-470-2100; Fax: 402-470-2345; Email: strydom@inetnebr.com). Journal of Biomolecular Techniques 11:185–186 © 2000 ABRF RF AB Feb 15–16 IBC’s Chemo * Bio Informatics, San Diego, CA. Contact: Jim Prudhomme, Lifesciences Marketing Division, IBC USA Conferences, Inc., 1 Research Drive, Suite 400A, Southborough, MA 01581. Tel: (508) 616-5550 X205; Fax: (508) 616-5533 (email: jprodhomme@ibcusa.com; URL: http://www.ibcusa.com/2621). Feb 20–23 WCBP 2001, 5th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Products, Washington, DC. Contact: Joan Oefner, Symposium Manager, Rhema Association Management, 156 South Spruce Avenue, Suite 207A, South San Francisco, CA 94080-4556; Tel: (650) 876-0792; Fax: (650) 876-0793 (email: joefner@casss.org; URL: http://www.casss.org/wcbp). Feb 24–27 ABRF 2001: The New Biology: Technologies for Resolving Macromolecular Communications, San Diego, CA. Contact: ABRF Meeting Management, FASEB, Office of Scientific Meetings, 9650 Rockville Pike, Bethesda, MD 20814. Tel: (301) 530-7010; Fax: (301) 530-7014 (email: marcella@faseb.org; URL: http://www.faseb.org/meetings/abrf2001/). Feb 26–28 IBC’s Enzyme Technologies 2001, San Diego, CA. Contact: Jim Prudhomme, Lifesciences Marketing Division, IBC USA Conferences, Inc., 1 Research Drive, Suite 400A, Southborough, MA 01581. Tel: (508) 616-5550 X205; Fax: (508) 616-5533 (email: JOURNAL OF BIOMOLECULAR TECHNIQUES, VOLUME 11, ISSUE 4, DECEMBER 2000 185
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Page 21 and 22: REFERENCES 1. Angeletti RH, Bonewal
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Page 33 and 34: BOOK REVIEWS Amino Acid Analysis Pr
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Page 37 and 38: AMINO ACID COMPOSITION AND SEQUENCE
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Page 45 and 46: P5-M Using high speed data collecti
Page 47 and 48: P13-S Microsatellite analysis using
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Page 57 and 58: P53-M Nano-LC ion-trap mass spectro
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Page 61 and 62: P69-T Efficient detergent and Cooma
Page 63 and 64: P77-M The utilities of N-terminal s
Page 65 and 66: P85-S Rapid oligosaccharide mapping
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