EBV Conference 2008 Guangzhou - Baylor College of Medicine

103 (RegID: 1282)
Ting Ting Yang
Institution: Department of Microbiology, Medical College of Qingdao University
e-mail: ttyang629@126.com
GENOTYPING OF EPSTEIN - BARR VIRUS IN EPSTEIN-BARR VIRUS ASSOCIATED
GASTRIC CARCINOMA
Yang Ting-ting,Wang Yun,Liu Xia,Li Xin,Pang Zeng-chang,Luo Bing
Posterabstract:
The fact that EBV infection is ubiquitous in the world but EBV associated malignant diseases incidence
differs in geographic regions raises the possibility that some subtype of EBV is involved in some kind of
tumor. We compare polymorphism of EBV among Epstein-Barr virus associated gastric carcinoma
(EBVaGC) and healthy adults in Shandong, which belongs to north of eastern China. Tumor samples from
236 gastric carcinoma cases and throat washings (TWs) from 135 healthy adults were collected and
screened for EBV genome. Then the 17 EBVaGCs and 33 EBV positive TWs were examined for type 1/2
EBV and polymorphism at BamHI F region, BamHI W1/I1 boundary region and XhoI restriction site in
LMP1 gene. Among them, neither of type 2 or type f of BamHI F polymorphism was found in EBVaGC
cases, nor type f in TWs. 25 of 33 TWs (75.8%) analyzed maintained type 1 virus, while 8 TWs (24.2%)
maintained type 2 virus. Type I and i of BamHI W1/I1 polymorphism accounted 1(5.9%) and 16(94.1%)
of EBVaGC and 11(33.3%) and 19(57.6%) of TWs, respectively. LMP1 XhoI(+) and (-) polymorphism
accounted 0(0) and 15(88.2%) of EBVaGCs and 12(36.4%) and 18(54.5%) of TWs, respectively. The
distribution rate of type i/XhoI(-) in EBVaGC(15/17, 88.2%) was higher than in TWs(5/33,15.2%). Our
findings show that type 2 virus is rare in EBVaGC. Compared with relative data from southern China, type
I and type f EBV strains are both to some extent geographically restricted polymorphism in China, with
their higher detection rate in southern China than in northern China. A distinctive EBV strain (i/XhoI(-)) is
associated with Chinese EBVaGC. It is interesting to find a candidate of specific tumor marker (XhoI (-))
for Chinese EBV related malignant diseases, as it was reported to be the predominant polymorphism in
Chinese nasopharyngeal carcinoma and Hodgkin’s disease.
EBV Conference 2008 Guangzhou
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