EBV Conference 2008 Guangzhou - Baylor College of Medicine

202 (RegID: 1221; 1222)
Chou Sheng-ping
Institution: National Health Research Institutes
e-mail:860812@nhri.org.tw
AN ALTERNATIVE FLUORESCENCE METHOD TO DETECT ANTIBODY AGAINST
EPSTEIN-BARR VIRUS DNASE, A USEFUL SEROMARKER IN SCREENING PATIENTS
WITH NASOPHARYNGEAL CARCINOMA
Sheng-Ping Chou, Mei-Ying Liu, Jen-Yang Chen
Posterabstract:
Human antibody which can neutralize Epstein-Barr virus (EBV) DNase activity has proven a useful
seromarker in screening patients with nasopharyngeal carcinoma (NPC). A conventional way to detect the
neutralizing antibody was done by DNase neutralization assay in which the DNA substrate needed to be
radiolabelled by 14C for sensitivity. However, this results in inconvenience in material preparation,
increase in isotope contamination risk and money cost. A better way to overcome these drawbacks was
created by applying PicoGreen fluorescence in the DNase neutralization assay. Optimal condition for the
PicoGreen-assay was set step by step and tested for 186 human sera which were also analyzed in parallel
by the 14C-assay. Comparing data from these two methods by Kappa Statistics, a Kappa coefficient of
0.818 was obtained and it indicates that the new PicoGreen-assay shows almost perfect in agreement with
the old 14C-assay. It is concluded that the easier, saver and cheaper PicoGreen-assay can replace the
traditional 14C-assay to detect antibody neutralizing EBV DNase activity and will be more applicable in
screening NPC patients.
EBV Conference 2008 Guangzhou
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