EBV Conference 2008 Guangzhou - Baylor College of Medicine

166 (RegID: 1751)
Bo Zhao
Institution: Brigham and Women's Hospital, Harvard Medical School
e-mail: bzhao@rics.bwh.harvard.edu
EBV NUCLEAR ANTIGENS EBNA3A AND EBNA3C REUGLATED CELLULAR GENES
B Zhao, J Mar, S Maruo, K Holton, E Johannsen, J Quackenbush, E Kieff
Posterabstract:
EBV nuclear proteins EBNA3A and EBNA3C are essential for EBV mediated B lymphocyte
transformation to lymphoblastoid cell lines (LCLs) in vitro. Both EBNA3A and 3C modulate the DNA
binding activity of cellular protein CSL and genetic analyses have shown that CSL/EBNA3A/C binding is
critical for the maintenance of continuous LCL growth. Thus EBNA3A and EBNA3C may contribute to
LCL growth by acting at CSL regulated promoters. To identify EBNA3A and 3C regulated cell genes,
LCLs were generated by transformation of PBMC with EBV recombinants that have EBNA3A or 3C
fused in frame to a mutant estrogen receptor hormone binding domain, which is activated by 4-hydroxy
tamoxifen. LCL growth and EBNA3AHT or EBNA3CHT expression were dependent on 4HT. Total cell
RNA were prepared from 3 independent clones of EBNA3AHT and EBNA3CHT LCLs that were grown
in the presence or absence of 4HT for 1, 2, 4 or 8 days. Changes in cell mRNAs have been identified using
Affymetrix U133 plus 2 oligonucleotide gene arrays. By Linear Models for Microarray Data (LIMMA)
analyses, 95 and 137 cell RNAs were differentially regulated by EBNA3A or 3C respectively during the
time course with P