EBV Conference 2008 Guangzhou - Baylor College of Medicine

44 (RegID: 1453)
Hideyuki Ishii
Institution: Asahikawa medical college
e-mail: ishii@asahikawa-med.ac.jp
THE INVESTIGATION OF INTERACTION BETWEEN NASAL NK/T-CELL LYMPHOMA AND
THE INFLAMMATION CELL.
Hideyuki Ishii, Miki Takahara, Yasuaki Harabuchi, Eva Klein
Oralabstract:
Background: Nasal NK/T-cell lymphoma (NNKTL) is the lymphoproliferative disorder with necrotic
granulomatous legions in the central part of head and neck region such as nasal cavity and hard palate. It
is known Epstein–Barr virus (EBV) associates with etiology of this lymphoma and is detectable in the
tumor cells.This lymphoma is characterized by the remarkable necrosis formation and the extensive
infiltration of inflammation cells in tumor tissue histopathologically. In terms of these, we investigate the
interactions between inflammation cells and NNKTL in this study.
Methods: Monocyte and granulocyte were separated from buffy coat of the volunteers as inflammation
cells. Each of these cells was co-cultured with SNK6 (NNKTL cell line) and KAI3 (EBV positive NK cell
line) without IL-2. Proliferation and LMP1 expression were compared with these lines (SNK6, KAI3)
without monocyte culture with/without IL-2. Finally, we investigated CD14 expression of 20 NNKTL
patients tissues by immunohistochemistry.
Results: CD14 expression was detected all NNKTL patient tissue. SNK6 and KAI3 showed the
proliferation under the condition that they co-cultured with monocyte without IL-2. LMP1 expression of
these cells increased at the same time. SNK6 and KAI3 did not show any proliferation under the condition
that they co-cultured with granulocyte without IL-2. LMP1 expression of these cells did not increase.
SNK6 and KAI3 did not show any proliferation under the condition that they co-cultured with monocyte
without cell-to-cell contact without IL-2. LMP1 expression of SNK6 and KAI3 did not increase at the
same time.
Conclusions: This study suggested that monocyte is one of the factors that influence to NNKTL
proliferation. It is possible for NNKTL to proliferate without IL-2, and cell-to-cell contact of monocyte
and NNKTL cell was needed to give rise to this phenomenon.
EBV Conference 2008 Guangzhou
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