EBV Conference 2008 Guangzhou - Baylor College of Medicine

28 (RegID: 1012)
Xuechi Lin
Institution: Cancer Research Institute, Xiangya School of Medicine, Central South University
e-mail: xuechilin71@126.com
REGULATION OF MAPK MEDIATED OP18/STATHMIN SIGNALING PATHWAY
TRIGGERED BY EBV ENCODED LMP1 IN NASOPHARYNGEAL CARCINOMA CELLS
Xuechi Lin, Sufang Liu, Lili Guo, Zijian Li, Xiaoqian Ma, Lili Li, Xiyun Deng, Ya Cao#
Oralabstract:
Op18/stathmin is a molecule proposed to relay integrating diverse cell signaling pathways, the regulation
of Op18/stahmin signaling can cause crucial changes of cellular biological properties including cell
growth and proliferation. Our studies focused on the regulation of Op18/stathmin signaling by LMP1 in
nasopharyngeal carcinoma (NPC) cells, Our results showed that LMP1 didn’t affect the expression of
Op18/stathmin , but LMP1 promoted the phosphorylation of Op18/stathmin. LMP1 was able to regulate
Op18/stathmin signaling through MAPK mediation, but the activity of MAPK induced by LMP1 was not
constant at different phases, changed with the cell cycle progression. LMP1 up-regulated the
phosphorylation of MAPK at G1/S. On the contrary, LMP1 negatively regulated the phosphorylation of
MAPK at G2/M phase. Inhibition of LMP1 expression attenuated the interaction of MAPK with
Op18/stathmin and promoted microtubule depolymerization. All these revealed a new pathway through
which LMP1 regulates Op18/stathmin signaling by MAPK mediation. The new signaling pathway on
LMP1 which is closely associated with cell cycle progression not only perfects the LMP1 regulation
network, but also provides new insights for elucidating the molecular mechanism of LMP1 leading to
tumorigenesis.
EBV Conference 2008 Guangzhou
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