Corynebacterium glutamicum - JUWEL - Forschungszentrum Jülich

G. Alternative Off-line Optimized Production Experiments
feed [L.h −1 ]
0.08
0.06
0.04
0.02
0
0 5 10 15 20 25
time [h]
0
0 5 10 15 20 25
time [h]
(a) Suggested experiment. Feed rates of a 500 (b) Expected results of the suggested experiment.
g/L glucose solution as a solid line and of a Biomass in OD600 as ×, glucose in mM as ∗ and
55 mM L-isoleucine solution as a dashed line. Ini- L-valine in mM as +.
tial concentrations were 50 g/L glucose, 10 mM
L-isoleucine and 4.60 µM pantothenic acid. No further
feed of pantothenic acid was needed.
Figure G.1.: Suggested experiment for a maximized TVP, without limitations with
respect to the biomass concentration.
G.2. Overall Yield of Product on Substrate
Optimization of the yield of product on substrate suffers from the risk of suggesting very
low final product concentrations because of the positive effect of using little substrate
for biomass production. Since this can not be a realistic optimal process, a penalty
function was introduced to prevent such very low final product concentrations. The
used optimization criterion was
ξ ∗ =argmax
ξ
Pend
Sused,int
biomass [OD 600 ], glc [mM], val [mM]
400
300
200
100
�
fs · (Pmin − Pend) : Pend