Physics And Chemistry Basis Of Biotechnology - De Cuyper - tiera.ru

Sheila J. Sadeghi et al
enzyme in the presence of the same molecules on the spectrophotometer. In all cases,
hydrogen peroxide formation due to uncoupling was minimal.
Pesticide mix PAH mix
Figure 12. Structure of the pesticides and PAHs screened for turnover by random mutants
of P450 BM3.
Similarly, the same assay was applied to investigate the interaction of various
compounds, including a number of polycyclic aromatic hydrocarbons (PAHs) and
pesticides (Figure 12), against a library of P450 BM3 variants, with random mutations
in the haem domain region. Two variants have been identified among about 320
screened, showing a significantly different pattern of turnover of certain of the
compounds compared to the wild type. Characterisation of the two active mutants is in
progress.
4. Designing a human/bacterial2El-BM3 P450 enzyme
P450 2E1 is a microsomal P450 present in the liver and other tissues of many
mammalian species that has been shown to catalyse the oxidation of over 80
compounds, including benzene, ethanol, acetone, chloroform, many nitrogenous
compounds together with drugs such as acetaminophen and chlorzoxazone. Having as
substrates ethanol and many suspect carcinogens, P450 2E1 has been considered of
great interest for its possible relevance to alcoholism, chemical carcinogenesis and
other diseases [84]. Its substrates have diverse structures but most of them have the
common characteristic of being low molecular weight molecules [ 146].
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