Physics And Chemistry Basis Of Biotechnology - De Cuyper - tiera.ru

3.2.1 . 1. Structure, fluidity and formation of s-BLMs ............................ 139
3.2.1.2. s-BLM with membrane proteins ..................................................141
3.2.2. Hybrid bilayer membranes (HBMs) ...................................................143
3.2.2.1. HBM as receptor surface and in immunological responses ... 144
3.2.2.2. HBM and membrane proteins ..................................................... 145
3.3. Selfassembled bilayers on solid or gel supports ........................................147
4 . s-BLMs with an aqueous reservoir trapped between the solid support and the
membrane ............................................................................................................ 150
4.1. Tethered lipid membranes ........................................................................ 150
4.2. Polymer cushioned bilayer lipid membranes ............................................ 154
5 . Phospholipid monolayers at the mercury/water interface, “Miller -Nelson
films” ................................................................................................................... 156
6 . Conclusions ..................................................................................................... 158
References ........................................................................................................... 159
Functional structure of the secretin receptor ............................................................ 167
P . Robberecht, M . Waelbroeck, and N . Moguilevsky ................................... 167
Abstract ................................................................................................................ 167
1 . Introduction .................................................................................................... 167
2 . The secretin receptor ...................................................................................... 168
2.1. General architecture .................................................................................. 168
2.2. Functional domains .................................................................................. 170
2.2.1. Ligand binding domain ...................................................................... 170
2.2.2. Coupling of the receptor to the G protein .......................................... 172
2.2.3. Desensitisation of the receptor ........................................................... 172
3 . Conclusions and perspectives ......................................................................... 172
Acknowledgements ..............................................................................................174
References ........................................................................................................... 174
Cold-adapted enzymes ............................................................................................. 177
D. Georlette, M. Bentahir, P. Claverie, T. Collins, S. D’amico, D. Delille.
G . Feller, E . Gratia, A . Hoyoux, T . Lonhienne, M-A . Meuwis, L . Zecchinon
and Ch . Gerday ................................................................................................... 177
1 . Introduction .................................................................................................... 178
2 . Enzymes and low temperatures ...................................................................... 178
3 . Cold-adaptation: generality and strategies ...................................................... 179
4 . Kinetic evolved-parameters ........................................................................ 180
5 . Activity/thermolability/flexibility .................................................................. 182
6 . Structural comparisons ................................................................................... 185
7 . Fundamental and biotechnological applications ............................................. 187
8 . Conclusions .................................................................................................... 189
Acknowledgements ............................................................................................. 190
References ........................................................................................................... 190
Molecular and cellular magnetic resonance contrast agents .................................... 197
J.W.M. Bulte and L.H. Bryant Jr ..................................................................... 197
Summary .............................................................................................................. 197
1 . Introduction .................................................................................................... 197
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