Physics And Chemistry Basis Of Biotechnology - De Cuyper - tiera.ru

2 . Magnetically labelled antibodies .................................................................... 198
3 . Other magnetically labelled ligands ............................................................... 200
4 . Magnetically labelled cells ............................................................................. 201
5 . Axonal and neuronal tracing ........................................................................... 203
6 . Imaging of gene expression and enzyme activity ........................................... 204
7 . Conclusions .................................................................................................... 206
References ........................................................................................................... 206
Radioactive microspheres for medical applications ................................................. 213
Urs Häfeli .........................................................................................................213
Summary ..............................................................................................................213
1. Definition of microspheres ..............................................................................213
2 . Applications and in vivo fate of microspheres ................................................214
3 . General properties of radioactive microspheres ..............................................217
3.1. Alpha-emitters .......................................................................................... 217
3.2. Beta-emitters ............................................................................................. 218
3.3. Gamma-emitters ....................................................................................... 220
4 . Preparation of radioactive microspheres .........................................................221
4.1. Radiolabeling during the microsphere preparation ................................... 222
4.2 Radiolabeling after the microsphere preparation ....................................... 224
4.3. Radiolabeling by neutron activation of pre-made microspheres ......... 226
4.4. In situ neutron capture therapy using non-radioactive microspheres ..228
5 . Diagnostic uses of radioactive microspheres ..................................................229
6 . Therapeutic uses of radioactive microspheres ................................................234
6.1. Therapy with alpha-emitting microspheres ...............................................234
6.2. Therapy with beta-emitting microspheres .................................................235
7 . Considerations for the use of radioactive microspheres ..................................240
References ............................................................................................................242
Radiation-induced bioradicals: ................................................................................ 249
Physical. chemical and biological aspects ............................................................... 249
Wim Mondelaers and Philippe Lahorte ........................................................... 249
Abstract ................................................................................................................249
1 . Introduction .................................................................................................... 249
2 . The interaction of ionising radiation with matter ............................................ 251
3 . The physical stage ........................................................................................... 254
3.1. Direct ionizing radiations ......................................................................... 254
3.2. Indirect ionizing radiations ....................................................................... 256
3.3. Linear Energy Transfer (LET) .................................................................. 257
3.4. Dose and dose equivalent ......................................................................... 257
3.5. Induced radioactivity ................................................................................ 258
4 . The physicochemical stage ............................................................................. 259
5 . The chemical stage ..........................................................................................261
5.1. Radical reactions with biomolecules ........................................................ 262
5.1 . 1. Radiation damage to DNA ...............................................................263
5.1.2. Radiation damage to proteins .............................................................265
5.1.3. Radiation damage to lipids and polysaccharides ................................ 266
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