Physics And Chemistry Basis Of Biotechnology - De Cuyper - tiera.ru

Summary ........................................................................................................... 47
1 . Synthesis ....................................................................................................... 47
1 . 1. Divergent ................................................................................................ 48
1.2. Convergent ............................................................................................. 49
1.3. Heteroatom ............................................................................................. 49
1.4. Solid phase .............................................................................................. 51
1.5. Other ........................................................................................................ 51
2 . Characterisation ............................................................................................. 52
3 . Biotechnology applications ........................................................................... 53
3.1. Biomolecules .......................................................................................... 53
3.2. Glycobiology .......................................................................................... 55
3.3. Peptide dendrimers ................................................................................. 56
3.4. Boron neutron capture therapy ............................................................... 57
3.5. MR imaging agents ................................................................................ 58
3.6. Metal encapsulation ............................................................................... 60
3.7. Transfection agents ................................................................................ 60
3.8. Dendritic box .......................................................................................... 62
4 . Concluding remarks ...................................................................................... 63
References ......................................................................................................... 63
Rational design of P450 enzymes for biotechnology ............................................. 71
Sheila J . Sadeghi. Georgia E . Tsotsou, Michael Fairhead. Yergalem T .
Meharenna and Gianfranco Gilardi .................................................................... 71
Abstract .............................................................................................................. 71
1 . Introduction ................................................................................................... 72
1 . 1. Interprotein Electron Transfer ................................................................ 72
1.2. Structure-function of cytochrome P450 enzymes .................................. 75
1.2.1. P450 redox chains ........................................................................... 75
1.2.2. P450 catalysis .................................................................................. 76
1.2.3. Bacterial P450s in biotechnology .................................................... 76
1.2.4. P450s in drug metabolism ............................................................... 78
1.3. Chimeras of P450 enzymes .................................................................... 79
1.3.1, Bacterial P450-P450-reductase fusion protein systems ................. 80
1.3.2. Plant P450-P450-reductase fusion proteins ..................................... 80
1.3.3. Plant/mammalian P450-P450-reductase fusion proteins ................ 80
1.3.4. Mammalian fusion proteins .............................................................. 81
1.4. Biosensing ............................................................................................... 81
2 . Engineering artificial redox chains ............................................................... 84
3 . Screening methods for P450 activity ............................................................ 90
3.1. Assay methods for P450-linked activity ................................................ 90
3.2. Development of a new high-through-put screening method for
NAD(P)H linked activity ........................................................................ 91
3.3. Validity of the new screening method .................................................... 93
4 . Designing a human/bacterial 2E1-BM3 P450 enzyme ................................. 94
4.1. Modelling ............................................................................................... 95
4.2. Construction ........................................................................................... 96
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