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Introduction au RNA-seq - Institut de Mathématiques de Toulouse

Introduction au RNA-seq - Institut de Mathématiques de Toulouse

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Préparation ation <strong>de</strong>s librairies<br />

ies<br />

<br />

Ligation <strong>de</strong>s adaptateurs (contiennent l’in<strong>de</strong>x)<br />

• P5 et P7: Fixation sur la flowcell<br />

• SP 1 et 2: primers <strong>de</strong> séquençage<br />

• Tag: in<strong>de</strong>x (6 bases): multiplexage par 24<br />

Enrichissement par PCR <strong>de</strong>s fragments+adaptateurs (Primers spécifiques <strong>de</strong> P5 et P7)<br />

<br />

Purification sur gel<br />

<br />

Contrôles qualités <strong>de</strong>s librairies : Bioanalyser, qPCR<br />

http://get.genotoul.fr

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