R - Lorentz JÄNTSCHI

Conclusion
- 107 -
Thin-layer chromatography was used to study the retention behavior of 14
newly synthesized s-triazines. The stationary phase used was silica gel with
covalently bonded C-18 phase (HPTLC) or silica gel impregnated with paraffin oil
(TLC), whith two-component mixtures of water and different organic solvents as
mobile phases.
On the basis of the obtained results the following conclusions can be drawn:
1. The retention behavior of the invesigated newly synthesized s-triazines is generaly
influenced by their molecular structure.
2. The retention is influenced by the polarity of the nature of the organic solvent
used as a mobile phase modifier in the case of the protic solvents, whereas the
retention is more or less the same with aprotic solvents.
3. Linear change of the retention with the change of volume ratio of the modifier in
the reverse phase was confirmed for the majority of tested chromatographic
systems. The exceptions were methanol on RP C-18 and acetonitrile on silica gel
impreganted with paraffin oil, for which a quadratic dependence was found.
4. High degree of mutual correlation between the retention parameters obtained for
the different mobile phases indicates the existence of compatibility of the
modifiers, and by the same token, it offers the posibility of optimization of the
retention of studied compounds.
5. Retention parameters obtained by the extrapolation to 0% of organic modifier,
RM 0 , represent a real measure of the lipohilicity of molecules, in contrast to the
programs for calculating logP values, which are not always equally valid and
reliable for expressing the partition coefficient.
Doktorska disertacija Lidija Jevrić