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16 Agriculture 7(2001)<br />

RESULTS AND DISCUSSION<br />

Average intensity of attack of ECB was 51.67% on all<br />

examined hybrids in the last ten years. The lowest attack<br />

was 24.17% in 1990. Results in 1991 were missing, due<br />

to war agression on our country. Since 1992 intensity of<br />

attack of ECB has been considerable every year (Table 1).<br />

Tablica 1. Intenzitet napada po godinama<br />

Table 1. Intensity of attack per years<br />

One of the reasons why intensity of attack increased<br />

could be because 35% in 1991 of corn was not harvested.<br />

Those conditions were favorable for ECB, and the attack<br />

was increased to 78.15% (Ivezi} & Raspudi}, 1993). In<br />

the same year, the hybrids BC 678, BC 66-61, BC 66-62,<br />

BC 778 and Ivan 31-81 had the intensity of attack of ECB<br />

100%, in 27 hybrids the attack was over 70%.<br />

Tolerance of particular hybrids to attack of ECB was<br />

also examined. Hybrid OSSK 552 was intolerant to ECB.<br />

Yield loss of 15.48% because of ECB attack was determined<br />

in several years’ trials in this hybrid. Hybrids OSSK<br />

664 and OSSK 554 had yield loss from 4-5%.The greatest<br />

loss in BC hybrids was determined in BC 66-61 (13.12%)<br />

and the lowest yield loss had BC 462 (0,82%) (Raspudi}<br />

et al., 1998).<br />

Differences in attack intensity occurred between different<br />

FAO groups. From 1992-1996, the lowest intensity<br />

of attack was in the FAO group 100 (34.25%), in subsequents<br />

FAO groups, the intensity was increased accordingly.<br />

The greatest intensity was in FAO 600, with<br />

71.76%. In all other groups, intensity varied from 45.83%<br />

to 66.01% (Ivezi} et al., 1997).<br />

Control of ECB has not been implemented in Croatia.<br />

There were just couple of field trials with biological preparation<br />

on the base of Bacillus thuringiensis, with only one<br />

application, which decreased intensity of attack by 9%<br />

(Valen~i} et al., 1988).<br />

Control of ECB with biological preparation Biobit XL,<br />

on the base of Bacillus thuringiensis, showed good results.<br />

Comparing to untreated plots the intensity of attack<br />

was 83 %, and on treated plots intensity decreased to 53%<br />

(for one application applied on July 10). Intensity of attack<br />

decreased to 35% in treatment with application implemented<br />

on July 28 and in treatments with two applications<br />

(July 10 and July 28) on 37%. Number of tunnels and larvae<br />

per corn plant also decreased, so Biobit XL seems to<br />

be efficient and ecologically acceptable preparation for<br />

ECB controlling (Ivezi} et al., 1998).<br />

In 1998 the first trials with GM corn plant were done<br />

in Croatia (Raspudi} et al., 1999). Field trials showed tolerances<br />

of GM corn to ECB attack (Table 2).<br />

Hybrid Evelina Bt was tolerant to ECB, and hybrid<br />

Landia Bt had 21% intensity of attack, while standard Landia<br />

had 98%. Number of tunnels and larvae was also decreased.<br />

Length of damages on corn plant on Landia Bt<br />

was lower 98% comparing to check. Decreasing in attack<br />

intensity in GM hybrids, caused better yield compared to<br />

standard hybrids. Yield increased by 10.27% in Evelina Bt,<br />

and for 26.67% in Landia Bt, comparing to their checks,<br />

respectively.<br />

Tablica 2. Razlika izme|u dva hibrida kukuruza i njihovih GM hibrida kukuruza<br />

Table 2. Differences between two corn hybrids and their genetically modified counterparts

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