Williams-Beureni sÃ¼ndroomi kromosoomi regiooni 22 valgu ...

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It is clear that WBSCR22 protein localizes in the nucleus but nobody has demonstrated that the predicted NLS at C-terminus of WBSCR22 protein is functional. Based on my study, I found that C-terminal domain is needed for nuclear transport giving free road to do further experiments, such as mutating NLS sequence and studying the effect of such mutations on the protein localization. It would also be interesting to fuse WBSCR22 NLS to the terminus of an unrelated protein, such as GFP, to see if the sequence is sufficient to target the protein to the nucleus. The final goal would be to determine the transport machinery that is involved in the transport of WBSCR22 to the cell nucleus, which would further help to elucidate WBSCR22 functions and possibly its role in the development of disease in humans. 26
KIRJANDUSE LOETELU 1. Bilanges B, Varrault A, Basyuk E, Rodriguez C, Mazumdar A, Pantaloni C, Bockaert J, Theillet C, Spengler D ja Journot L (1999). Loss of expression of the candidate tumor suppressor gene ZAC in breast cancer cell lines and primary tumors. Oncogene 18, 3979–3988. 2. Cheng X ja Roberts RJ (2001). AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucleic Acids Research 29(18), 3784-3795. 3. Daniel JA, Pray-Grant MG ja Grant PA (2005). Effector proteins for methylated histones: an expanding family. Cell Cycle 4, 919–926. 4. Doll A. ja Grzeschik KH (2001). Characterization of two novel genes, WBSCR20 and WBSCR22, deleted in Williams-Beuren syndrome. Cytogenet Cell Genet 95:20– 27. 5. Gao SB, Feng ZJ, Xu B, Wu Y, Yin P, Yang Y, Hua X ja Jin GH (2009). Suppression of lung adenocarcinoma through menin and polycomb gene-mediated repression of growth factor pleiotrophin. Oncogene 28, 4095– 4104. 6. Gorlich D ja Kutay U (1999). Transport between the cell nucleus and the cytoplasm. Annu Reb Cell Dev Biol 15, 607-660. 7. Hoelz A, Debler EW ja Blobel G (2011). The Structure of the Nuclear Pore Complex. Annual Review of Biochemistry 80, 20.1–20.31 8. Huang SM, Schonthal AH ja Stallcup MR (2001). Enhancement of p53-dependent gene activation by the transcriptional coactivator Zac1. Oncogene 20, 2134–43. 9. Järvinen-Pasley A, Bellugi U, Reilly J, Mills DL, Galaburda A, Reiss AL ja Korenberg JR (2008). Defining the social phenotype in Williams syndrome: A model for linking gene, the brain, and behavior. Development and Psychopathology 20 , 1– 35. 10. Kalderon D, Roberts BL, Richardson WD, Smith AE (1984). A short amino acid sequence able to specify nuclear location. Cell 39, 499–509. 11. Kalderon D, William DR, Alexander FM ja Alan ES (1984). Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311, 33 – 38. 12. Kim JY, Kee HJ, Choe NW, Kim SM, Eom GH, Baek HJ, Kook H, Kook H ja Seo SB (2008). Multiplemyeloma-related WHSC1/MMSET isoform RE-IIBP is a 27
Page 1 and 2: TARTU ÜLIKOOL LOODUS- JA TEHNOLOOG
Page 3 and 4: KASUTATUD LÜHENDID DAPI - 4’,6-d
Page 5 and 6: 1. KIRJANDUSE ÜLEVAADE 1.1 William
Page 7 and 8: 320 kb. Noolte abil on näidatud LC
Page 9 and 10: EIF4H LAT2 RFC2 CLIP2 GTF2IRD1 WBSC
Page 11 and 12: Edasised uuringud näitasid, et Mer
Page 13 and 14: sel moel, et ta seostub otse tuuma
Page 15 and 16: 2.2 Materjal ja metoodika 2.2.1 Pla
Page 17 and 18: 2.2.4 Western blot analüüs 24 tun
Page 19 and 20: 2.3 Tulemused ja arutelu 2.3.1 WBSC
Page 21 and 22: antikeha kui ka kommertsiaalset pol
Page 23 and 24: IF III - WBSCR22NTD DAPI Anti-WBSCR
Page 25: RESÜMEE Cellular localization of W
Page 29 and 30: wingless protein and is in the Will

Williams-Beureni sÃ¼ndroomi kromosoomi regiooni 22 valgu ...

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