TARTU ÜLIKOOL

SISUKORDSisukord .......................................................................................................................... 2Lühendid ........................................................................................................................ 3Sissejuhatus .................................................................................................................... 41. KIRJANDUSE ÜLEVAADE ..................................................................................... 51.1. Tupe mikrofloora .................................................................................................. 51.1.1. Koostis ......................................................................................................... 51.1.2. Tähtsus ........................................................................................................ 61.1.3. Mõjutavad tegurid ....................................................................................... 71.1.4. Korüneformsed bakterid kui tupe mikrofloora komponent ......................... 81.1.4.1. Korüneformsete bakterite morfoloogia ja klassifikatsioon ......... 81.1.4.2. Liigiline koostis ja hulgad tupes .................................................. 91.2. Meessuguteede mikrofloora ................................................................................. 91.2.1. Meessuguteede normaalne mikrofloora..................................................... 101.2.2. Meessuguteede mikrofloora prostatiidi korral ........................................... 101.2.3. Korüneformsed bakterid kui meessuguteede mikrofloora komponent ..... 112. EKSPERIMENTAALOSA ...................................................................................... 122.1. Töö eesmärgid .................................................................................................. 122.2. Materjal ja meetodid ....................................................................................... 122.2.1. Uuritavad mehed ja naised ........................................................................ 122.2.2. Uuritavad korüneformsete bakterite tüved .............................................. 132.2.3. Korüneformsete bakterite samastamine ................................................... 142.2.4. Statistiline analüüs .................................................................................... 142.3. Tulemused ja arutelu ..................................................................................... 152.3.1. Korüneformsed bakterid tupes.................................................................... 152.3.2. Korüneformsed bakterid spermas tervetel ja prostatiidiga meestel ............ 162.3.3. Korüneformsed bakterid prostatiidiga meeste ning kontrolluuritavatenaispartneritel ...................................................................................................... 172.3.4. Erinevate meetodite võrdlus suguteede korüneformsete bakteritesamastamisel ....................................................................................................... 182.3.4.1. Erinevate praimerite võrdlus ..................................................... 182.3.4.2. Sekveneerimise ja API meetodil samastamise võrdlus ............ 20KOKKUVÕTE .............................................................................................................. 22RESÜMEE/SUMMARY ............................................................................................... 23TÄNUAVALDUSED ..................................................................................................... 25KASUTATUD KIRJANDUS ........................................................................................ 26LISAD ............................................................................................................................ 302