Untersuchungen zur Kapazitation und Hyperaktivierung equiner ...

Ergebnisse
and acrosome damaged cells (FITC-PNA positive; closed gray squares) are plotted
as a function of the incubation time under capacitating conditions. Averages ±
standard deviations of three ejaculates are presented.
Figure 4. In vitro capacitation properties for stallion sperm incubated in Tyrode A
capacitation medium (A) or Tyrode B control medium (B). Percentages of plasma and
acrosomal membrane intact cells (PI negative/FITC-PNA negative; closed black
squares), plasma membrane damaged cells (PI positive; open squares), and
acrosome damaged cells (FITC-PNA positive; closed gray squares) are plotted as a
function of the incubation time. Averages ± standard deviations of three ejaculates of
each of five stallions are presented.
Figure 5. In vitro capacitation properties for stallion sperm incubated in Whittens A
capacitation medium (A-B) or Whittens B control medium (C-D), in the absence (A,
C) and presence (B, D) of 5 mM procaine. Percentages of plasma and acrosomal
membrane intact viable cells (PI negative/FITC-PNA negative; closed black squares),
plasma membrane damaged cells (PI positive; open squares), and acrosome
damaged cells (FITC-PNA positive; closed gray squares) are plotted as a function of
the incubation time. Averages ± standard deviations of one ejaculate of each of five
stallions are presented.
Figure 6. Curve line velocity (A) and amplitude of lateral head movement (B) for
stallion sperm incubated in Whittens A capacitation medium (closed symbols) or
Whittens B control medium (open symbols), for 90 min, in the absence (squares) and
41