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M A R K T Ü B E R S I C H T<br />
Fig. 5: Multi-photon<br />
cross-sections for<br />
some fluorophores*<br />
and 3D image processing and measurement<br />
packages called LaserPix and Laser-<br />
Vox respectively. There are special features<br />
such as co-localisation analysis and<br />
volumetric object measurements. All three<br />
packages read the Bio-Rad PIC file format.<br />
Expert Support<br />
Bio-Rad offers Technical and Applications<br />
support and provides innovative tools<br />
for customer use such as the interactive<br />
fluorescence database on Fluorescence.<br />
bio-rad.com<br />
Microscope adaptablity means that you<br />
are not limited to one type of microscope<br />
or set of objective lenses.<br />
AOTF or ND for controlling laser power<br />
Flyback blanking to reduce photobleaching<br />
The increased scan speeds and ROI scanning<br />
allow FRAP and timecourse experiments.<br />
The sequential line by line (Lamba Strobing)<br />
scanning mode collects multi-channel<br />
images almost as fast as simultaneous<br />
scanning, but without any bleed-through<br />
problems.<br />
The new Bi-directional scanning mode<br />
allows data to be collected while the laser<br />
beam is scanning in both directions, thus<br />
enabling faster data collection<br />
Photon Counting up to 30 photons per<br />
pixel per scan dramatically increases signal<br />
to noise with an excellent linearity of<br />
response.<br />
Prismatically enhanced PMTs for<br />
increased sensitivity<br />
Optimised transmitted light DIC – optical<br />
fibers retain laser beam polarity.<br />
Independent confocal irises allow iris optimisation<br />
for different emission wavelengths<br />
or for the same ‘optical section’<br />
to be collected into each PMT. This is not<br />
possible with single or fixed pinholes during<br />
simultaneous imaging.<br />
Digital Mixers allow real-time bleedthrough<br />
correction.<br />
External triggering allows scanning to be<br />
triggered from an external electronic signal<br />
Beam Conditioning unit enables optimum<br />
filling of lens back aperture. Beam recolli-<br />
Trademarks<br />
SYTO ® Alexa Fluor ® Bodipy ® Fluo-3 FM1-43 DiI DiO<br />
(Molecular Probes, Inc. Eugene, Oregon, USA)<br />
EBFP, CFP, GFP, YFP, DsRed Living Colors ® Fluorescent proteins<br />
(Clontech Laboratories Inc. Palo Alto, California, USA)<br />
Cy2, Cy3 and Cy5 are all Trademarks of Amersham International<br />
PLC © 1995<br />
Radiance2100, LaserSharp 2000, LaserPix and Laser-<br />
Vox (Bio-Rad Microscience Ltd).<br />
Acknowlegements<br />
Figure1: Photograph by Craig Michhelli (Micchelli et al. (1997) Development<br />
124, 1485-1495. Reproduced with permissin from S.<br />
Blair and the Conpany of Biologists<br />
Figure 2: SA Endow and DJ Komma, Duke University, NC, USA<br />
Figure 4: J. Mc Cormick et al., University of Michigen, USA<br />
Figure 5: Watt Webb, Cornell University<br />
mation device corrects the chromatic shift<br />
between visible and infrared lasers.<br />
Software options<br />
Bio-Rad offers a data acquisition software<br />
called LaserSharp 2000 as well as 2D<br />
Tab. 1: Applications with the new Radiance2100 series<br />
Confocal<br />
Lasers Wavelengths Application 1 Applications 2 Applications 3<br />
Argon ion 457 CFP Lucifer Yellow Fixed DAPI<br />
Chlorophyll.Alexa430<br />
477 wtGFP less excitation of<br />
YFP than 488<br />
488 EGFP standard green fluors. Fluo-3 calcium green<br />
514 EYFP Syto11, 15, in vivo FM1-43 memebrane dye<br />
nuclear stains<br />
Krypton/Argon 488 EGFP DsRed standard green red Cy2, FITC, Alexa 488,<br />
double labelling double labelling DiO<br />
568 DsRed standard red fluors. RxRed, Lissamine<br />
Green HeNe 543 Cy3 Tritc standard orange/red RH-414<br />
Propidium<br />
Dil<br />
Red Diode 638 Cy5 Allophycocyanin<br />
Options<br />
Blue Diode 406 CFP/YFP DAPI Hoechst<br />
FRET Bimane<br />
BFP • wtGFP<br />
HeCAD 442 CFP/YFP Lucifer Yellow, Lyso<br />
FRET Green chlorophyll, Alexa 430<br />
Krypton 568 DsRed Texas Red & standard<br />
red Fluors., Alexa 568<br />
Multiphoton<br />
Spectra 680-1050 DAPI FITC, Tritc Serotonin (3-photon)<br />
Physics<br />
Hoechst<br />
Tsumani<br />
Bimane<br />
CFP/YFP, FRET<br />
CoherentMira 690-1000 as above<br />
Spectra 750-850 or standard green &<br />
Physics Mai 780-920 orange fluors.<br />
Tai<br />
CFP/YFP, FRET<br />
Coherent 700-760 DAPI Hoechst<br />
Vitesse XT<br />
Bimane<br />
760-860 DAPI Hoechst, Lucifer Yellow<br />
Bimane<br />
CFP/YFP, FRET<br />
780-920 as above FITC<br />
Coherent 800 DAPI Hoechst, wtGFP<br />
Vitesse<br />
Bimane,<br />
CFP/YFP, FRET<br />
Contact<br />
Dr. Hans Pankau<br />
BIO-RAD GmbH<br />
Tel.: 06723-913655<br />
Fax: 06723-913656<br />
eMail: Hans_Pankau@Bio-Rad.com<br />
28 | Nr. II/2001 |transkript LABORWELT
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