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PRODUCTION Of NUTRIENT SOURCES FOR RHIZOBIUM

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Table 5. (continued).<br />

Sugar alcohols Structure Description<br />

Mannitol<br />

( D -mannitol,<br />

Mammite)<br />

H H OH OH<br />

HOH 2C- C- C- C- C-CH 2OH<br />

OH OH H H<br />

Source : Shallenberger (1993); Christain (1994); Voet (1995).<br />

��<br />

The sugar alcohol, mannitol, is naturally<br />

occuring and is found in exudates of olive<br />

and plane trees, and in seaweed. It may be<br />

prepared by the reduction of mannose. The<br />

crystals melt at 166-168°C. The L-form is<br />

known, but does not occur in nature.<br />

(a) equilibrate thermodynamically with the solution by losing water and perhaps by admitting some of<br />

the extracellular solute(s), in which case it had died or become dormant, or (b) suffered a temporary<br />

loss of water but used energy to accumulated a solute(s) to a concentration thermodynamically<br />

commensurated with the extracellular a w. The solute can be a metabolite which was retained or an<br />

extracellular substance which was accumulated. The effect of solution accumulation was to lower<br />

intracellular a w values to the extended that water again entered the cell to achieved thermodynamic<br />

parity with the outside. Thermodynamic parity normally included a factor for an appropriated level of<br />

turgor pressure.<br />

1.4 Physiology of xerotolerant microorganisms<br />

The term “osmophilic” was introduced in 1912 (Brown, 1978). It has been useful but it is<br />

misleading, firstly because of the connotations which osmotic pressure can have, and secondly<br />

because its suffix, “-philic”, implies a requirement for, rather than a tolerance of, a concentrated<br />

growth medium (Brown, 1976, quoted in Brown, 1978). Sugar- and salt-tolerance can be used in

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