PRODUCTION Of NUTRIENT SOURCES FOR RHIZOBIUM

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Table 10. (continued). No. Isolate Culture medium Cell lysate No. Isolate Culture medium Cell lysate number Gly a Man b Gly a Man b number Gly a Man b Gly a Man b 51 LLY 1 ++ - - - 76 PAY4 ND ND ND ND 52 LLY 2 ++ - - - 77 PIY 1 +++ - - - 53 LLY 3 ++ - - ++ 78 PIY 2 ++ ++ +++ ++ 54 LLY 4 +++ - - - 79 PIY 3 +++ - - - 55 MAY 1 +++ - - - 80 POY 1 + - - - 56 MAY 2 +++ - - - 81 POY 2 ++ - - - 57 MEY 1 +++ - - - 82 POY 3 +++ - - - 58 MEY 2 +++ - - - 83 PUY 1 ++ - - - 59 MEY 4 +++ - - - 84 PUY 2 ++ - - - 60 MEY 3 +++ - - - 85 PUY 3 +++ - - - 61 MIY1 ND ND ND ND 86 PUY 4 +++ - + ++ 62 MLY 1 ++ - - - 87 RIY 1 +++ - - - 63 MLY 2 + - - - 88 RIY2 ND ND ND ND 64 MLY 3 +++ - - - 89 RIY 3 +++ - - - 65 OAY 1 + - - - 90 RIY4 ND ND ND ND 66 OAY 2 +++ - - - 91 RIY5 ND ND ND ND 67 OAY 3 +++ - - - 92 RIY 6 ND ND ND ND 68 OAY 4 + - - - 93 RIY7 ND ND ND ND 69 ORY 1 +++ - - - 94 SAY 1 +++ - - - 70 ORY 2 +++ - - - 95 SCY 1 +++ - - - 71 ORY 3 +++ - - - 96 SCY2 ND ND ND ND 72 ORY 4 +++ - - - 97 SCY3 ND ND ND ND 73 PAY 1 ++ - - - 98 SLY 1 +++ - + ++ 74 PAY 2 +++ - - - 99 SLY 2 +++ - - + 75 PAY 3 NG NG NG NG 100 SOY 1 + - - - ��
Table 10. (continued). No. Isolate Culture medium Cell lysate No. Isolate Culture medium Cell lysate number Glya Manb Glya Manb number Glya Manb Glya Manb 101 SOY 2 ++ - - - 125 SYY 2 +++ - - - 102 SOY 3 +++ - - - 126 SYY 3 ++ - - - 103 SOY4 ++ - - - 127 TAY 1 + - - ++ 104 SOY 5 + - - - 128 TAY 2 +++ - - - 105 SOY 6 NG NG NG NG 129 TAY 3 ++ - - - 106 SOY 7 NG NG NG NG 130 TAY 4 ++ - - - 107 SPY 1 +++ - - - 131 TAY 5 +++ - - - 108 SPY 2 - - - - 132 TAY 6 + - - - 109 SMY 1 +++ - - - 133 TAY 7 +++ - - ++ 110 SMY 2 - - - - 134 TAY 8 +++ - - - 111 SMY 3 + - - + 135 TAY 9 ++++ - - ++ 112 STY 1 +++ - - - 136 WAY 1 ++ - - - 113 STY 2 - - - - 137 WAY 2 ++ - - - 114 STY 3 NG NG NG NG 138 WAY 3 ++ - - - 115 STY 4 +++ - - - 139 WAY 4 + - - - 116 STY 5 - - - - 140 WAY 5 NG NG NG NG 117 STY 6 - - - - 141 WAY 6 + ++ - ++ 118 SUY 1 +++ - - - 142 VEY 1 +++ - - - 119 SUY 2 + - - - 143 YOY 1 + - - 120 SWY 1 ++++ - - - 144 Y 64 +++ - - - 121 SWY 2 +++ - - - 145 Y 24 NG NG NG NG 122 SWY 3 +++ - - - 146 Y 60 + - - + 123 SWY 4 ++ - - - 147 Y 69 +++ - - - 124 SYY 1 +++ - - - ND= not detected, NG= not growth, Gly a = Glycerol, Man b = Mannitol. ++++ = Dark brown, +++ = Medium brown, ++ = pale brown, + = very slightly, and - = no spot. ��
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PRODUCTION OF NUTRIENT SOURCES FOR
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PRODUCTION OF NUTRIENT SOURCES FOR
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CHARDCHAI BUROM : PRODUCTION OF NUT
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CONTENTS Page ABSTRACT (THAI)……
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3.6.1� Determination of the suita
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�� 17� Glycerol concent
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40� Time courses of mannitol prod
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59� Effects of various heat shock
Page 17 and 18:
LIST OF TABLES Table Page ��
Page 19 and 20:
LIST OF ABBREVIATIONS Aw water avai
Page 21 and 22:
CHAPTER I INTRODUCTION For mass cul
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available (Burton, 1965). The molec
Page 25 and 26:
1.1.1� Carbon metabolism in Rhizo
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strains unable to use the carbon so
Page 29 and 30: Figure 2. The fructose bisphosphate
Page 31 and 32: 1.2�Classification of yeasts Mank
Page 33 and 34: Table 3. (continued). Class Order F
Page 35 and 36: Ustilaginales (Oberwinkler, 1987).
Page 37 and 38: f Cystofilobasidium has thick-walle
Page 39 and 40: Table 5. (continued). Sugar alcohol
Page 41 and 42: specific cases, but Brown (1978) pr
Page 43 and 44: KY 6166 was also studied using larg
Page 45 and 46: Figure 5. Important pathways of gly
Page 47 and 48: may be present in different ratios.
Page 49 and 50: amylase systems of the yeast specie
Page 51 and 52: Table 7. (continued). EC Recommende
Page 53 and 54: Table 8. (continued). Species a D.p
Page 55 and 56: Table 9. (continued). Cell product
Page 57 and 58: 2.1.3 Equipment and other materials
Page 59 and 60: Spotted either the supernatant of c
Page 61 and 62: amylase action, the actual reaction
Page 63 and 64: A) Determination of the suitable an
Page 65 and 66: B) Salt-stress conditions Fifty mil
Page 67 and 68: 2.2.8 Cultivation of Bradyrhizobium
Page 69 and 70: The cultured medium of yeast. Quant
Page 71 and 72: intervals for accumulation of gas i
Page 73 and 74: CHAPTER III RESULTS AND DISCUSSION
Page 75 and 76: Glycerol Xylitol Glucose Mannitol S
Page 77 and 78: Glycerol Xylitol Glucose Mannitol S
Page 79: Table 10. Primary screening of glyc
Page 83 and 84: Glycerol in cell lysate (g/l) A Gly
Page 85 and 86: average were 4-10 g of glycerol per
Page 87 and 88: were isolated from fresh fruits, Ma
Page 89 and 90: �third day of incubation (Figure
Page 91 and 92: Reducing sugars (g/l) Reducing suga
Page 93 and 94: These results indicated that the co
Page 95 and 96: 3.6.1 Determination of the suitable
Page 97 and 98: g/l 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4
Page 99 and 100: Reducing sugars (g/l) 8 6 4 2 0 0 1
Page 101 and 102: Reducing sugars (g/l) 4.0 3.5 3.0 2
Page 103 and 104: g/l 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0
Page 105 and 106: g/l g/l g/l 1.5 1.0 0.5 0.0 1.2 1.0
Page 107 and 108: Starch fermentation was conducted i
Page 109 and 110: g/l g/l 7 6 5 4 3 2 1 0 8 6 4 2 0 0
Page 111 and 112: g/l g/l 8 6 4 2 0 8 6 4 2 0 1 2 3 4
Page 113 and 114: g/l g/l 8 6 4 2 0 8 6 4 2 0 0 1 1 2
Page 115 and 116: g/l g/l 1.6 1.4 1.2 1.0 0.8 0.6 0.4
Page 117 and 118: g/l g/l 1.6 1.4 1.2 1.0 0.8 0.6 0.4
Page 119 and 120: The results showed that amounts var
Page 121 and 122: g/l g/l 4.0 3.5 3.0 2.5 2.0 1.5 1.0
Page 123 and 124: �� fermentation, but the inc
Page 125 and 126: g/l g/l 2.0 1.6 1.2 0.8 0.4 0.0 3.2
Page 127 and 128: g/l g/l 1.2 1.0 0.8 0.6 0.4 0.2 0.0
Page 129 and 130: �� intracellularly a decreas
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g/l g/l 5.0 4.0 3.0 2.0 1.0 0.0 2.5
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g/l g/l 2 1.8 1.6 1.4 1.2 1 0.8 0.6
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g/L 7 6 5 4 3 2 1 0 1 2 3 4 5 6 7 0
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g/l 8 7 6 5 4 3 2 1 0 1 2 3 4 5 6 7
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�� effected by heat shock po
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Table 15. The comparison of media f
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Table 17. Growth of Bradyrhizobium
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�� Rhizobium (Stowers, 1985;
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�� 3.9 Identification of yea
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KAY1 PIY2 PUY4 Y69 LIY2 COY1 FAY2 C
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�� assimilations were observ
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Table 20. (continued). Yeast strain
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Table 20. (continued). 15. Pichia k
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�� 3.9.5 Ascospore formation
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(a) (b) (c ) (d) (e) �� Figu
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(a) (b) (c) (d) (e) (f) �� F
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(c) (a) �� Figure 72. Cell m
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�� Metshnikowia (Pitt and Mi
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CHAPTER IV CONCLUSION This study ex
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only a low capital investment. Ther
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�� Barnett, J. A., Payne, R.
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�� De Mot, R., and Verachter
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�� Jennings, D. H. (1995). T
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�� Lie, T. A., Muilenbury M.
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�� Onishi, H., and Suzuki, T
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�� Sinclair, N. A., and Stok
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�� Van Rossum, D., Schuurman
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�� Young, J. P. W., and Hauk
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Acetone Methanol Solvent for mobile
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� 300 µg/ml, 200 µg/ml, and 100
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Soluble Starch 20.0 g Distilled wat
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13.�Nitrate broth KNO 3 1.0 g Glu
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If the sample had been diluted befo
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Table 1D. (continued). Source of ye
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Page 207 and 208:
Page 209 and 210:
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E. Additional figures Abs 540 nm (a
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Abs 580 nm 1.4 1.2 1 0.8 0.6 0.4 0.
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Figure 7E. Calibration curves for H
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Figure 9E. Example of HPLC chromato
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Cell/mL 1.0E+09 8.0E+08 6.0E+08 4.0
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PRODUCTION Of NUTRIENT SOURCES FOR RHIZOBIUM

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