Ben-Gurion University of the Negev

3.4.4.2. Autumn
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In green leaves, the flavonoid content of peak #3 was the highest, 4.24 ± 0.26 mg/gFW,
while that of quercetin-3-O-glucoside was the lowest, 0.48 ± 0.01 mg/gFW (Table 6).
In red leaves, the flavonoid content of peak #3 was the highest, 3.97 ± 0.36 mg/gFW,
while those of cyanidin-3-O-glucoside and quercetin-3-O-glucoside were the lowest,
0.86 ± 0.19 and 0.68 ± 0.23 mg/gFW, respectively and did not differ significantly of
each other (P>0.05) (Table 6).
Table 6. Flavonoids content and retention times in green and red
leaves of P. atlantica. Flavonoid content is expressed as mg/g
fresh weight. The results are expressed as mean±SD
Peak no. 1 2 3 4 5
Peak
identity
Retention
time
(min.)
Harvest
Spring
green
Spring
red
Autumn
green
Autumn
N.I.
Cyanidin-
3-Oglucoside
N.I.
2.71±0.08 8.49±0.78 8.92±0.76
Quercetin-
3-Oglucoside
11.28±1.0
4
N.I.
12.28±0.0
9
5.68±0.69 3.43±0.52 5.45±1.04 1.70±0.45 5.09±1.80
(a*) (b,c) (a,b) (c) (a,c)
4.31±0.35 3.84±0.73 5.55±1.69 2.79±0.53 11.61±2.1
(A) (A,B) (A,B) (B) 2 (C)
2.91±0.47
(k)
1.06±0.01
(l)
4.24±0.26
(m)
0.48±0.01
(n)
-
red
2.53±0.17
(K)
0.86±0.19
(L)
3.97±0.36
(M)
0.68±0.23
(L)
-
* For each harvest, means bearing the same letter are not significantly
different according to t-test (P>0.05); N.I. - not identified; Unidentified
peaks, #1, 3 and 5 are expressed as cyanidin-3-O-glucoside equivalents;
Peaks #2 and 4 contents are expressed according to their own standard curves
The ratios of the combined relative peak areas of the characteristic peaks (#; 1, 2, 3, 4
and 5) to the total peaks area were 0.31 ± 0.02 in green leaves and 0.21 ± 0.01 in red
leaves (Appendix, Table 2).