Ben-Gurion University of the Negev

44
Table 8. Flavonoids content and retention times in green and red leaves of P.
lentiscus. Flavonoid content is expressed as mg/g fresh weight. The results are
expressed as mean±SD
Peak no. 1 2 3 4 5 6
Peak
identity
Retention
time
(min.)
Harvest
Winter
(Cyprus
ecotype)
Winter
(Tunisian
ecotype)
Spring
(Cyprus
ecotype)
Autumn
(Cyprus
ecotype)
N.I.
Cyanidin-
3-Oglucoside
N.I.
Quercetin-
3-Oglucoside
N.I. Cyanidin
2.62±0.03 7.32±1.55 7.71±1.55 9.51±1.40 10.88±0.86 12.86±0.80
1.82±0.58
(a*)
1.59±0.97
(A,B)
1.30±0.65
(k,m)
0.62±0.07
(K)
5.81±0.31
(b)
6.39±1.84
(B)
12.60±2.78
(l)
4.78±1.37
(L)
3.55±1.43
(a,b)
4.47±1.96
(B)
9.39±1.10
(l)
4.06±1.23
(L)
1.97±0.53
(a)
2.89±0.62
(A,B)
3.56±0.72
(m)
1.47±0.16
(L)
1.99±0.23
(a)
2.13±0.05
(A,B)
3.15±1.07
(m)
0.98±0.47
(K,L)
3.21±0.70
(a)
4.71±0.10
(B)
5.14±0.74
(m)
1.59±0.41
(K,L)
* For each harvest, means bearing the same letter are not significantly different according to ttest
(P>0.05); N.I. - not identified; Unidentified peaks, #1, 3 and 5 are expressed as cyanidin-3-
O-glucoside equivalents; Peaks #2, 4 and 6 contents are expressed according to their own
standard curves
3.4.6.1.2. Spring
Highest, and similar, flavonoid contents were those of cyanidin-3-O-glucoside and peak
#3, 12.60 ± 2.78 and 9.39 ± 1.10 mg/gFW, respectively, while those of peaks #1 and 5
were the lowest, 1.30 ± 0.65 and 3.15 ± 1.07 mg/gFW, respectively (Table 8).
Similar ratios of the combined relative areas of the characteristic peaks (#; 1, 2, 3, 4, 5
and 6) to the total peaks area of the Cyprus ecotype were determined, about 0.78 ± 0.03
(Appendix, Table 2).
3.4.6.1.3. Autumn
In the Cyprus ecotype, highest flavonoid contents were those of cyanidin-3-O-glucoside
and peak #3, 4.78 ± 1.37 and 4.06 ± 1.23 mg/gFW, respectively (Table 8). These peaks
did not differ significantly (P>0.05). In contrast, peaks #1 and 5 had the lowest
flavonoid contents, 0.62 ± 0.07 and 0.98 ± 0.47 mg/gFW, respectively (Table 8).