Full Journal - Journal of Cell and Molecular Biology - Haliç Üniversitesi

70 Tülin Aktaç and Elvan Bakar
Material and methods
The adult mice (Balb/C, Albino), raised at
Experimental Medicine Research and Application
Center-University of ‹stanbul were used. Animals
were divided by four groups (3 experiment and 1
control) for experiments. Each group contained three
animals. Different concentrations of AlCl3 (0.1 %, 1
% and 5 %) prepared in drinking water were given to
experiment groups while only drinking water was
given to the control group. The amounts of water
consumed by the animals during the experiment are
shown in Table 1. Animals were killed by cervical
dislocation at the day 10. For the ligth microscopic
examinations, tiroid glands were fixed in formalin (10
% solution) for 24 hours and then embedded in
paraffin wax. Sections of 5 µm thick were cut and
stained with hematoxylin-eosin.
Table 1: The amounts of water consumed by the animals
during the experiment.
A1C13 doses Animal no. Water amounts (ml)
0.1 % 1 20
2 18
3 22
1 % 1 15
2 12
3 10
5 % 1 21
2 18
3 16
Control 1 22
2 17
3 20
Results
The results of present study showed that progressive
degenerative changes in thyroid gland were depended
on the dose of aluminium. There was no significantly
degenerative changes in the groups of 0.1% and 1%
AlCl3 compared the control group. It was observed
that the general histological structure of the gland was
disappeared in some sections (Figure 1-3).
The most abundant degenerative changes were
observed in 5 % AlCl3 group. In the thyroid follicles,
destruction, distortions of thyroglobulin were
Figure 1: The thyroid tissues of control group. f: follicles,
c: colloid, s: stroma (interstitiel tissue), bar representes
20 µm.
Figure 2: 0.1% AlCl3 group. The general histological
structure of the gland was disappeared, bar representes
20 µm.
Figure 3: 1% AlCl3 group. The general histological
structure of the gland was disappeared, bar representes
10 µm.