Full Journal - Journal of Cell and Molecular Biology - Haliç Üniversitesi

90 Gülruh Ulako¤lu
According to these results 2, 4 and 6 mg/ml
concentrations of ADM 24 hours after administration
of EAT cells, all concentrations caused a quite
definite cytotoxic effect in comparison to the control,
the most effective concentration was 6 mg/ml. The
cytotoxic effect at 24 hours continued at after 30
hours and after 48 hours the cytotoxic effect of all the
concentrations increased. According to this
observation, ADM induces a cytotoxic effect on EAT
cells depending on concentration and time.
Discussion
EAT cells survive in acidic fluid present in the
peritoneal cavity of mice in the form of a suspension.
It was known that, after the transplantation into
peritoneal cavity of mice the number of cells increase
exponentially up to the 9. day and reach the
plato-phase following 9. and 10. days (Lazebnik et al.,
1991; Song et al., 1993; Vinuela et al., 1991). For this
reason, during the in vivo experiments of this study
ADM was injected to mice at the 4. day following
EAT transplantation to them. The reason why ADM
was given in the middle of the multiplication period 4.
day to see how it affected the exponential growth of
EAT cells.
In a study similar to this one, to DBA/2 mice
bearing acidic murine lymphocytic leukemia, ADM
and daunorubicin were administered separately and
their survival periods were controlled. In this study
0.25-2.00 mg/kg ADM was injected to tumor bearing
mice for 5 days. The survival of mice, receiving a
high dose prolonged 140 % that of the controls and 20
% of the experimental mice survived up to to 50. day
(Schwartz and Grindey, 1973). Even though different
kind of tumor bearing mice and different
concentrations of ADM were used, the results of this
experiment also supports theirs.
Experiments on the survival time’s prolongation
started in 1978. Extracts prepared from muscle and
liver tissues of mice were injected i.p. for a period of
12 days to EAT bearing mice. It was found that these
mice survived longer that the control ones. In
contrast, a positive results were not obtained from
spleen, kidney, lung, serum, skin and small intestine
extracts (Tong et al., 1978).
Different doses of ADM was investigated in
various cells. It was shown that ADM in 0.2 mg/ml
concentration exerted cytotoxic effect on human
peripheral lymphocytes. In the same study, it was also
observed that this concentration decreased the
multiplication of V79 (Chinese hamster’s lung
fibroblast cells) cells 50 % (Szabona, 1996). In our
study, it was also observed that ADM’s 2, 4 and 6
mg/ml concentrations exerted a cytotoxic effect on
EAT cells which increased depending on time. When
K562 (human erythroleukemia) cell, propagated in
tissue culture, were treated with 5, 10 and 30 mM
ADM, it was determined that the cell number was
decreased in comparison to the control and the drug
exerted a cytotoxic effect depending on the dose used
(Ciaccia et al., 1994). It was observed that allogenic
tumor cells and mouse spleen cells, propagated in cell
culture, when treated with ADM in an in vitro
medium, ADM exerted a cytotoxic effect on them
(Ehrke et al., 1984; Tomozic et al., 1980). It was also
found that when HeLa cells, propagated in an in vitro
medium, were treated with 5, 10, 25, 50 and 100
mg/ml ADM, their survival ratio decreased depending
on the dose (Jagetia and Nayak, 1996).
Even though ADM makes cytotoxic effect in
tumor cells, it also exerts negative effects on normal
cells. For this reason, when ADM was administered to
patients in the clinic, drugs which remove its side
effects were also used (Vichi and Tritton, 1993).
Many studies are present where ADM is applied in
various doses to many different kinds of tumors. In
this study, the results derived from first time this
ADM doses applied to EAT cells are in accordance
with those indicated in literature.
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