Full Journal - Journal of Cell and Molecular Biology - Haliç Üniversitesi

88 Gülruh Ulako¤lu
et al., 1993). A group of research workers, treated
their breast carcinoma, gastric carcinoma and colon
carcinoma patients with a single dose of 25 mg/m 2 i.v.
ADM. After peripheral blood samples were obtained
serially from these patients before and after drug
injection were compared. They found that ADM had
a cytotoxic effect on the blood cells which was its side
effect (Arinaga et al., 1986).
In this study, whether ADM prolonged or not the
survival periods of mice bearing EAT cells was
investigated. Besides the mode of effect of ADM’s
different doses on EAT cells multiplication was also
examined in vitro.
Material and methods
EAT cells used in this study were hyperdiploid
carcinoma cells, and they were maintained in our
laboratory by making their transplantions every 7
days.
Preparation of ADM
ADM isolated from Streptomyces peucetius by
Farmitalia Research Laboratories for the first time.
ADM (Carlo Erba, Turkey) used in the experiments
was a lyophylized and stock solution obtained by
dissolving 10 mg ADM in 5 ml sterile water. From
this solution 2, 4, 6 mg/ml ADM concentrations were
prepared to be used in the experiments.
In vivo experiments
In the experiments 3 months old male albino mice of
strain Balb/c weighing between 25-30 g were used.
The mice were first inoculated intraperitonally with
3X10 6 EAT cells and they were separated into control
and experimental groups. There are 10 mice in the
control group and 29 mice in the experimental group.
In the in vivo experiment were used total 39 mice. 4
days after the inoculation of EAT cells, 10 mg/g ADM
was injected i.p. in a single dose to the experimental
mice. The control group’s mice were injected with
physiological serum. The status of all the mice were
controlled every day and the death dates of both the
experimental and control mice were determined.
In vitro experiments
In these experimental series also EAT cells were
drawn from the stock mice with an injector and a cell
suspension was prepared in BSS. From this
suspension, 3X10 5 cells/ml were transferred into steril
tubes containing medium (Eagle + 2.5% Fetal calf
serum + 10 mM Hepes). After the tubes were closed
with steril rubber stoppers, they were incubated in an
incubator at 37°C. EAT cells were separated into
experimental and control tubes, 24 hours after being
taken in vitro condition. ADM in 2, 4 and 6 mg/ml
concentrations were added separately into the
experimental tubes (Çerçi and Ulako¤lu, 2000). At
the end of 3, 5, 24, 30 and 48 hours of drug addition,
cell counted were done. Both the control and
experimental cells were treated with trypan blue,
counted under the light microscope and the amount of
dead and surviving cells were determined.
According to the data obtained, by the application
of paired t-test their significance at p