"Development of microbial treatment of ret liquor generated in a coir ...

More documents

Recommendations

Info

CHAPTER-2 DEVELOPMENT OF MICROBIAL CONSORTIA FOR RET LIQUOR TREATMENT 2.1. INTRODUCTION For the development of ret liquor treatment systems an assemblage of microorganisms capable enough to degrade the polyphenols, proteins, lipids, carbohydrates, cellulose, hemicellulose and pectins in ret liquor, generated during the retting process, is required. It was quite appropriate to make a search for such organisms in the retting ground itself as due to years together of the retting of the retting there could have happened segregation, colonisation, and even mutation of the type of organisms which can cleave the fairly resistant polyphenols. Before isolating such organisms the samples were subjected for selective enrichment in ret liquor to eliminate the used organisms. This chapter deals with the procedure for the isolation of such organisms followed and the description oforganisms obtained. 11
2.2 MATERIALS AND METHODS For generating appropriate microbial consortia towards the envisaged ret liquor treatment system three types of enrichment procedures were executed as follows: 2.2.1. Successive enrichment in husk infusion 2.2.1a Preparation of husk infusion Husk infusion was prepared by boiling 500g freshly split husk in 20ppt seawater for 30 minutes. The supematant was decanted off and , the salinity determined and corrected to 20ppt by adding distilled water. For preparing the enrichment medium 225ml husk infusion was supplemented with NHtCl (2gm) , NH 4 NO] (0.5gms) and yeast autolysate (250mg) and altogether autoclaved at 15 lbs for l Smin, The system was completed by inoculating the medium with 25ml sample prepared out of sediment obtained from a retting ground at Chellanum north. The sediment sample (20gm) from the above retting ground was suspended in 20ppt sterile seawater (IOOml) and agitated vigorously for 5 minutes. After allowing the sediment to settle for a brief period 25ml supematant was inoculated in 225ml husk infusion medium. The preparation after adjusting the pH to 7 was 12
Page 1 and 2: DEVELOPMENT OF MICROBIAL TREATMENT
Page 5 and 6: ACKNOWLEDGEMENTS I sincerely dedica
Page 7: I also thank all my colleagues Mr.T
Page 10 and 11: 2.3. RESULTS AND DISCUSSIONS 37 2.3
Page 12: CHAPTER -1 GENERAL INTRODUCTION
Page 15 and 16: 'mallis' and dumped in water. Rocky
Page 17 and 18: activity has been attributed to bac
Page 19 and 20: habitat, for oysters, clams and mus
Page 22 and 23: along with the once generated by th
Page 26 and 27: incubated over magnetic stirrer and
Page 28 and 29: 2.2.2. Isolation of heterotrophic b
Page 31 and 32: The above medium was aseptically di
Page 33: polyphenol content in each flask wa
Page 36: output in each case. A culture was
Page 39 and 40: The plates were then flooded with G
Page 41 and 42: The medium was autoclaved at 10lbs
Page 43: 2.2.8.d. Isolation of organisms Two
Page 46 and 47: after adjusting pH t07.5±O.2 and a
Page 48 and 49: 2.2.9.b. Isolation and segregation
Page 51 and 52: energy sources and their percentage
Page 54 and 55: In a similar manner from 7 samples
Page 56: media were segregated. These proced
Page 59 and 60: Table 2.1. Monitoring an enrichment
Page 62 and 63: Table 2.5 Enrichment, isolation, pr
Page 64: Table 2.6. Secondary screening of t
Page 68 and 69: Table 2.9. Enrichment, isolation, p
Page 70 and 71: Table 2.11. Enrichment, isolation,
Page 72 and 73: Table 2.13. Bacterial Cultures fina
Page 76 and 77:
CHAPTER 3 DEVELOPMENT OF ACTIVATED
Page 78 and 79:
Once in 24 hours the pH was adjuste
Page 80:
a hot plate and kept boiling gently
Page 84 and 85:
3.3 RESULTS AND DISCUSSION For the
Page 87:
On comparing the oxygen consumption
Page 90:
ultimately implemented by individua
Page 98 and 99:
CHAPTER-4 PACKED BED REACTOR
Page 100 and 101:
point 36 cm from the base an inlet
Page 102 and 103:
4.2.3. Inoculating the packed bed w
Page 104 and 105:
the process repeated without any ad
Page 106 and 107:
adsorption of cel1 on to the shel1
Page 108 and 109:
order to persist in this environmen
Page 111 and 112:
FigA.2 100 90 80 70 c 0 ..... a. E
Page 113 and 114:
c: 0 100 90 80 70 .... a- 60 E ::>
Page 118 and 119:
• In a similar manner from the ab
Page 120 and 121:
• There existed a positive correl
Page 122 and 123:
• This type of circulation takes
Page 124:
LEGEND 1. Magnetic coupled pump 2.
Page 127 and 128:
REFERENCES Adamson , A.W. 1982. phy
Page 129 and 130:
Buchnan, R.E., and N.E Gibbons. 197
Page 131 and 132:
Fowler G.J., and Marsden F.J. 1925,
Page 133 and 134:
Marshall,K.C. 1985. Bacterial adhes
Page 135 and 136:
Pike, E.B., and C.R.Curds, 1971. Th
show all

"Development of microbial treatment of ret liquor generated in a coir ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?