"Development of microbial treatment of ret liquor generated in a coir ...

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2.2.9.b. Isolation and segregation From the treated ret liquor and the filtrant grams of the above enrichment system bacteria and fungi were isolated through ret liquor solidified with agar medium. Ret liquor prepared in 20ppt seawater was supplemented with 1% Nl-laCl, 0.2% NH4N03, 0.00 I% Yeast autolysate and for the isolation of bacteria the pH was adjusted to 7.5 and for that of fungi the pH was brought down to 4.8 using IN NaOH and tartaric acid respectively. The medium was solidified with 2% agar and the polyphenol in the medium was around Ippm.The plates made out of these media were swabbed with diluted samples oftreated ret liquor and filtrant grains. Dilution ofthe samples were made in 20ppt seawater aseptically. Plates were incubated at 28±OA IJC for 7days. Isolation From each sample bacterial and fungal culture were isolated into nutrient agar and Sabouraud dextrose agar slants prepared in ret liquor base as described under section 2.2.8.d 35
Segregation of potent strains As described under section 2.2.8.e. the cultures were subjected for primary screening in ret liquor supplemented with NH 4Cl,NH 4N03 and Yeast autolysate identified to genera and subjected to secondary screening in ret liquor without any addition of nutrients. Those which exhibited fairly higher percentage ofpolyphenoI consumption were segregated and subjected for further characterization as described under the section 2.2.7.a t02.2.7.g Maintenance ofcultures The segregated bacterial and fungal cultures were maintained in nutrient agar and Sabouraud dextrose agar vials prepared in ret liquor (20ppt) and one set was overlaid with sterile liquid paraffin and the other sealed with paraffin wax. 36
Page 1 and 2: DEVELOPMENT OF MICROBIAL TREATMENT
Page 5 and 6: ACKNOWLEDGEMENTS I sincerely dedica
Page 7: I also thank all my colleagues Mr.T
Page 10 and 11: 2.3. RESULTS AND DISCUSSIONS 37 2.3
Page 12: CHAPTER -1 GENERAL INTRODUCTION
Page 15 and 16: 'mallis' and dumped in water. Rocky
Page 17 and 18: activity has been attributed to bac
Page 19 and 20: habitat, for oysters, clams and mus
Page 22 and 23: along with the once generated by th
Page 24 and 25: CHAPTER-2 DEVELOPMENT OF MICROBIAL
Page 26 and 27: incubated over magnetic stirrer and
Page 28 and 29: 2.2.2. Isolation of heterotrophic b
Page 31 and 32: The above medium was aseptically di
Page 33: polyphenol content in each flask wa
Page 36: output in each case. A culture was
Page 39 and 40: The plates were then flooded with G
Page 41 and 42: The medium was autoclaved at 10lbs
Page 43: 2.2.8.d. Isolation of organisms Two
Page 46 and 47: after adjusting pH t07.5±O.2 and a
Page 51 and 52: energy sources and their percentage
Page 54 and 55: In a similar manner from 7 samples
Page 56: media were segregated. These proced
Page 59 and 60: Table 2.1. Monitoring an enrichment
Page 62 and 63: Table 2.5 Enrichment, isolation, pr
Page 64: Table 2.6. Secondary screening of t
Page 68 and 69: Table 2.9. Enrichment, isolation, p
Page 70 and 71: Table 2.11. Enrichment, isolation,
Page 72 and 73: Table 2.13. Bacterial Cultures fina
Page 76 and 77: CHAPTER 3 DEVELOPMENT OF ACTIVATED
Page 78 and 79: Once in 24 hours the pH was adjuste
Page 80: a hot plate and kept boiling gently
Page 84 and 85: 3.3 RESULTS AND DISCUSSION For the
Page 87: On comparing the oxygen consumption
Page 90: ultimately implemented by individua
Page 98 and 99:
CHAPTER-4 PACKED BED REACTOR
Page 100 and 101:
point 36 cm from the base an inlet
Page 102 and 103:
4.2.3. Inoculating the packed bed w
Page 104 and 105:
the process repeated without any ad
Page 106 and 107:
adsorption of cel1 on to the shel1
Page 108 and 109:
order to persist in this environmen
Page 111 and 112:
FigA.2 100 90 80 70 c 0 ..... a. E
Page 113 and 114:
c: 0 100 90 80 70 .... a- 60 E ::>
Page 118 and 119:
• In a similar manner from the ab
Page 120 and 121:
• There existed a positive correl
Page 122 and 123:
• This type of circulation takes
Page 124:
LEGEND 1. Magnetic coupled pump 2.
Page 127 and 128:
REFERENCES Adamson , A.W. 1982. phy
Page 129 and 130:
Buchnan, R.E., and N.E Gibbons. 197
Page 131 and 132:
Fowler G.J., and Marsden F.J. 1925,
Page 133 and 134:
Marshall,K.C. 1985. Bacterial adhes
Page 135 and 136:
Pike, E.B., and C.R.Curds, 1971. Th
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