"Development of microbial treatment of ret liquor generated in a coir ...

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2.2.2. Isolation of heterotrophic bacteria from the enrichment systems The cultures developed in the husk infusion was serially diluted at every stage of subculturing / passage with 20ppt aged seawater as diluent and inoculated by pour plate method on to nutrient agar prepared in 20ppt aged sea water, husk infusion prepared with 20ppt aged seawater, and also in Sabouraud dextrose prepared in husk infusion. Nutrient Agar (NA) Peptone Beef extract Yeast extract 20 ppt aged seawater Agar PH Husk Infusion Nutrient agar (HINA) 0.5g 0.5g O.lg 100ml 2.0g Peptone Beefextract Yeast extract Husk infusion prepared in 20ppt sea water 15 7.5 + 0.1 0.5g 0.5g O.lg 100ml
Agar pH Husk Infusion Sabouraud Dextrose Agar Dextrose Peptone Husk infusion in 20ppt sea water Agar pH 2.0g 7.5+0.1 2.0g l.Og 100ml 2.0g 4.8 pH was adjusted by adding 4ml tartaric acid after autoclaving the medium at 10 Ibs for 10 min. The plates were incubated at 28±OAoC for 7 days and 20 bacterial colonies from each sample and medium were isolated onto respective medium slants .Altogether 180 colonies were isolated which were subsequently purified and stocked in media vials overlaid with sterile liquid paraffin for further study.(Fungi were not found to have developed on husk infusion Sabouraud dextrose agar plates.) 16
Page 1 and 2: DEVELOPMENT OF MICROBIAL TREATMENT
Page 5 and 6: ACKNOWLEDGEMENTS I sincerely dedica
Page 7: I also thank all my colleagues Mr.T
Page 10 and 11: 2.3. RESULTS AND DISCUSSIONS 37 2.3
Page 12: CHAPTER -1 GENERAL INTRODUCTION
Page 15 and 16: 'mallis' and dumped in water. Rocky
Page 17 and 18: activity has been attributed to bac
Page 19 and 20: habitat, for oysters, clams and mus
Page 22 and 23: along with the once generated by th
Page 24 and 25: CHAPTER-2 DEVELOPMENT OF MICROBIAL
Page 26 and 27: incubated over magnetic stirrer and
Page 31 and 32: The above medium was aseptically di
Page 33: polyphenol content in each flask wa
Page 36: output in each case. A culture was
Page 39 and 40: The plates were then flooded with G
Page 41 and 42: The medium was autoclaved at 10lbs
Page 43: 2.2.8.d. Isolation of organisms Two
Page 46 and 47: after adjusting pH t07.5±O.2 and a
Page 48 and 49: 2.2.9.b. Isolation and segregation
Page 51 and 52: energy sources and their percentage
Page 54 and 55: In a similar manner from 7 samples
Page 56: media were segregated. These proced
Page 59 and 60: Table 2.1. Monitoring an enrichment
Page 62 and 63: Table 2.5 Enrichment, isolation, pr
Page 64: Table 2.6. Secondary screening of t
Page 68 and 69: Table 2.9. Enrichment, isolation, p
Page 70 and 71: Table 2.11. Enrichment, isolation,
Page 72 and 73: Table 2.13. Bacterial Cultures fina
Page 76 and 77: CHAPTER 3 DEVELOPMENT OF ACTIVATED
Page 78 and 79:
Once in 24 hours the pH was adjuste
Page 80:
a hot plate and kept boiling gently
Page 84 and 85:
3.3 RESULTS AND DISCUSSION For the
Page 87:
On comparing the oxygen consumption
Page 90:
ultimately implemented by individua
Page 98 and 99:
CHAPTER-4 PACKED BED REACTOR
Page 100 and 101:
point 36 cm from the base an inlet
Page 102 and 103:
4.2.3. Inoculating the packed bed w
Page 104 and 105:
the process repeated without any ad
Page 106 and 107:
adsorption of cel1 on to the shel1
Page 108 and 109:
order to persist in this environmen
Page 111 and 112:
FigA.2 100 90 80 70 c 0 ..... a. E
Page 113 and 114:
c: 0 100 90 80 70 .... a- 60 E ::>
Page 118 and 119:
• In a similar manner from the ab
Page 120 and 121:
• There existed a positive correl
Page 122 and 123:
• This type of circulation takes
Page 124:
LEGEND 1. Magnetic coupled pump 2.
Page 127 and 128:
REFERENCES Adamson , A.W. 1982. phy
Page 129 and 130:
Buchnan, R.E., and N.E Gibbons. 197
Page 131 and 132:
Fowler G.J., and Marsden F.J. 1925,
Page 133 and 134:
Marshall,K.C. 1985. Bacterial adhes
Page 135 and 136:
Pike, E.B., and C.R.Curds, 1971. Th
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