"Development of microbial treatment of ret liquor generated in a coir ...

was filtered and stored at 20 DC till used. Hundred ml aliquots were
autoclaved in 250ml conical flasks at 10 Ibs 10 min. The flasks were
inoculated from a nutrient agar slant grown culture and incubated at
28±OA DC for 12 hours as static cultures. Controls included uninoculated
husk infusion incubated under the same conditions. Initial and final
polyphenol content was estimated following the methods described under
section 2.2.I.d
2.2.5 Determination of the percentage consumption /degradation of
polyphenols by the selected cultures.
Tannic acid was used as the substrate in this determination. A 0.5%
tannic acid solution was prepared in the mineral base medium (for
composition see section 2.2.3) and divided into aliquots of 20ml each in
100ml conical flasks. They were autoclaved at 10 lbs for 10 min. and
inoculated from a bacterial suspension of having 0.5 O.D prepared in
mineral base medium. In all cases one loopfull ofthe culture was inoculated
uniformly after determining the initial polyphenol content. The preparation
was incubated at room temperature over rotary shaker at 100 rpm. At 24 th ,
48 th , nnd,120 t \ 40 th , 168 th and 192 nd hours of incubation the available
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