"Development of microbial treatment of ret liquor generated in a coir ...

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2.2.7.e. Lipolysis Tween compounds are generally used as the substrate for detecting the hydrolysis of fats. In addition to tween, the medium must contain a soluble calcium salt so that the released fatty acid is then turned to insoluble calcium salt around the colony. The medium contained the following ingredients. Peptone csci, Tween 80 NaCI Agar Distilled water pH 109 O.lg 109 20g 20g 1000ml 7.2±0.2 The medium was autoc1aved and poured into plates and the organisms are spot inoculated and incubated at 28±OAoC for 7days. Opaque zone surrounding microbial growth consisted of calcium salts of free fatty acid and are usually taken as being indicative ofa positive lipolytic activity. 2.2.7.£ . Amylolysis Amylase production was checked in nutrient agar supplemented with 2% starch. The medium was autoc1aved and poured into plates and spot inoculated and incubated for 72 hours. 25
The plates were then flooded with Gram's iodine Grams iodine Iodine KI Distilled water l.Og 2.0g 100ml Amylase producing organisms showed clearing zone around them and the colour of the zones depended on the degree of the hydrolysis of starch. The zones with reddish brown indicated that the starch was hydrolysed to dextrin and when the hydrolysis further continued the zone became glassy. Amylolysis was ascribed to both these reactions. 2.2.7.g. Pectinolysis Ability of microorganisms to grow in a defined medium containing pectin or pectic acid as the sole source carbon is not a reliable criterion of their ability to degrade pectic substances. This is largely because most commercially available pectins are chemically complex and can undergo further changes during sterilization (Jayasankar and Graharn, I970). Therefore to determine whether the organisms are pectinolytic in addition to testing their ability to utilize it as the sole source of carbon and energy an 26
Page 1 and 2: DEVELOPMENT OF MICROBIAL TREATMENT
Page 5 and 6: ACKNOWLEDGEMENTS I sincerely dedica
Page 7: I also thank all my colleagues Mr.T
Page 10 and 11: 2.3. RESULTS AND DISCUSSIONS 37 2.3
Page 12: CHAPTER -1 GENERAL INTRODUCTION
Page 15 and 16: 'mallis' and dumped in water. Rocky
Page 17 and 18: activity has been attributed to bac
Page 19 and 20: habitat, for oysters, clams and mus
Page 22 and 23: along with the once generated by th
Page 24 and 25: CHAPTER-2 DEVELOPMENT OF MICROBIAL
Page 26 and 27: incubated over magnetic stirrer and
Page 28 and 29: 2.2.2. Isolation of heterotrophic b
Page 31 and 32: The above medium was aseptically di
Page 33: polyphenol content in each flask wa
Page 36: output in each case. A culture was
Page 41 and 42: The medium was autoclaved at 10lbs
Page 43: 2.2.8.d. Isolation of organisms Two
Page 46 and 47: after adjusting pH t07.5±O.2 and a
Page 48 and 49: 2.2.9.b. Isolation and segregation
Page 51 and 52: energy sources and their percentage
Page 54 and 55: In a similar manner from 7 samples
Page 56: media were segregated. These proced
Page 59 and 60: Table 2.1. Monitoring an enrichment
Page 62 and 63: Table 2.5 Enrichment, isolation, pr
Page 64: Table 2.6. Secondary screening of t
Page 68 and 69: Table 2.9. Enrichment, isolation, p
Page 70 and 71: Table 2.11. Enrichment, isolation,
Page 72 and 73: Table 2.13. Bacterial Cultures fina
Page 76 and 77: CHAPTER 3 DEVELOPMENT OF ACTIVATED
Page 78 and 79: Once in 24 hours the pH was adjuste
Page 80: a hot plate and kept boiling gently
Page 84 and 85: 3.3 RESULTS AND DISCUSSION For the
Page 87:
On comparing the oxygen consumption
Page 90:
ultimately implemented by individua
Page 98 and 99:
CHAPTER-4 PACKED BED REACTOR
Page 100 and 101:
point 36 cm from the base an inlet
Page 102 and 103:
4.2.3. Inoculating the packed bed w
Page 104 and 105:
the process repeated without any ad
Page 106 and 107:
adsorption of cel1 on to the shel1
Page 108 and 109:
order to persist in this environmen
Page 111 and 112:
FigA.2 100 90 80 70 c 0 ..... a. E
Page 113 and 114:
c: 0 100 90 80 70 .... a- 60 E ::>
Page 118 and 119:
• In a similar manner from the ab
Page 120 and 121:
• There existed a positive correl
Page 122 and 123:
• This type of circulation takes
Page 124:
LEGEND 1. Magnetic coupled pump 2.
Page 127 and 128:
REFERENCES Adamson , A.W. 1982. phy
Page 129 and 130:
Buchnan, R.E., and N.E Gibbons. 197
Page 131 and 132:
Fowler G.J., and Marsden F.J. 1925,
Page 133 and 134:
Marshall,K.C. 1985. Bacterial adhes
Page 135 and 136:
Pike, E.B., and C.R.Curds, 1971. Th
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