"Development of microbial treatment of ret liquor generated in a coir ...

was used. The above test medium was taken at 10 ml aliquots in test tubes
and autoclaved at 10 lbs for l Omin, The tubes were inoculated from the
bacterial and fungal slant cultures after determining the initial polyphenol
content following the colourimetric method as described under section
2.2.1.d. Freshly grown bacterial cultures were then harvested in 20ppt
seawater and inoculated individually to obtain a final OD of 0.05 at 600nm.
In the case of fungi, the slants were incubated till sporulation took place and
harvested in 20ppt seawater and inoculated to obtain a final spore count of
10 2.mrl • Controls included uninoculated set of tubes.All tubes were
uniformly incubated at 28±OAoC for l Odays and the final polyphenol content
was estimated for every tube following the above mentioned method. From
the values the percent consumption ofpolyphenol in every tube was worked
out. The (>25%) were segregated and identified to genera following
Buchanan and Gibbons(1974)and Oliver(1982). This formed the primary
screening ofpolyphenoI consuming organisms.
Secondary screening
The above segregated cultures were subjected for secondary
screening, using ret liquor without any supplemented nutrients. Ret liquor
prepared as described above was transferred in l Oml aliquots into test tubes
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