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Dottorato in Scienze Biotecnologiche – XXI ciclo Indirizzo Biotecnologie Industriali Università di Napoli Federico II BIOCHIP AND LAB-ON-CHIP FOR GENOMIC AND PROTEOMIC APPLICATIONS Lucia Rotiroti Dottoranda: Rotiroti Lucia Relatore: Dr. De Stefano Luca Coordinatore: Prof. Giovanni Sannia
Page 1: BIOCHIP AND LAB-ON-CHIP FOR GENOMIC
Page 7 and 8: INDICE SUMMARY pag. i RIASSUNTO Cha
Page 9 and 10: RIASSUNTO Una delle più importanti
Page 11 and 12: molecolare, la chimica delle superf
Page 13 and 14: dell’ordine dei 10-100 nm. Questo
Page 15 and 16: Un caso particolarmente interessant
Page 17 and 18: concentrazione aumenta la bagnabili
Page 19 and 20: Reflectivity (a.u.) 1.4 1.2 1.0 0.8
Page 21 and 22: Di fondamentale importanza è la sc
Page 23: Chapter 1 Porous Silicon
Page 26 and 27: Figure 1: schematic of a biosensor
Page 28 and 29: In the past two decades, the biolog
Page 30 and 31: A simplified mechanism for the chem
Page 32 and 33: After the etching, the next step to
Page 34 and 35: Materials and Methods: Porous Silic
Page 36 and 37: The morphology of the porous silico
Page 38 and 39: Reflectivity (u.a) 1.0 0.8 0.6 0.4
Page 40 and 41: Thue-Morse: aperiodic multilayers m
Page 42 and 43: phys. stat. sol. (c) 4, No. 6 (2007
Page 44 and 45: phys. stat. sol. (c) 4, No. 6 (2007
Page 46 and 47: Optical sensing of chemical compoun
Page 48 and 49: line which slope is the optical pat
Page 50 and 51: 1942 L. De Stefano et al.: Quantita
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n 1944 L. De Stefano et al.: Quanti
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Chapter 2 Chemical and Biological f
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Thermal hydrosilylation provides a
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10. Robins EG, Stewart MP, Buriak J
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Sensors 2007, 7, 214-221 Full Resea
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Sensors 2007, 6 Table 1. PSi layers
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Sensors 2007, 6 The characteristic
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Sensors 2007, 6 The sensor response
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Examples of optical biosensors for
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esearch articles De Stefano et al.
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esearch articles De Stefano et al.
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INSTITUTE OF PHYSICS PUBLISHING JOU
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Nanostructured biosensors S2021 in
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Nanostructured biosensors S2023 A B
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Nanostructured biosensors S2025 Fig
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Nanostructured biosensors S2027 5.
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Optical evidence of protein structu
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ellipsometry (Horiba-Jobin-Yvon, mo
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concentration variations of 0.022 (
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Figure 6: (a) Simulated reflectivit
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Oligonucleotides direct synthesis o
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Porous silicon-based optical biosen
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In this work, we review our recent
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In Fig. 2, the red shifts of the m
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Chapter 3 Hybrid systems based on P
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Schematic of a PSi device as etched
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Porous silicon-polymer composite ma
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Introduction Due to their widesprea
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Organic compound Refractive index I
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Bio/NON Bio Interfaces for a new cl
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5 10 15 20 25 30 35 40 45 50 55 Pro
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5 10 15 20 25 30 35 aspecific bindi
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Chapter 4 …towards the Lab-on-Chi
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Figure 1: (1) An example of bulk mi
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Figure 4: Different types of bondin
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30. Camon H and Moktadir Z 1997 Sim
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Sensors 2006, 6, 680-687 Full Resea
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Sensors 2006, 6 682 From the optica
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Sensors 2006, 6 684 Intensity (a.u.
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Sensors 2006, 6 686 chamber and on
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Laser oxidation micropatterning of
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383 single components of a complex
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Fig. 2: functionalization steps of
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387 [3] R. Herino, G. Bomchil, K. B
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J20. L. De Stefano, A. Vitale, I. R
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C2. Luca De Stefano, F. G. Della Co
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C35. L. Rotiroti, E. De Tommasi, I.
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INSTITUTE OF PHYSICS PUBLISHING JOU
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LDeStefanoet al Reflectance 40 35 3
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LDeStefanoet al [2] De Stefano L, M
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J. Phys.: Condens. Matter 19 (2007)
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Page 164 and 165:
Page 166 and 167:
Extremophiles DOI 10.1007/s00792-00
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Extremophiles setup, used to measur
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Extremophiles ∆λ (nm) 1.4 1.2 1.
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Sensors 2008 2 analytes present in
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Sensors 2008 4 Figure 2. Schematic
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Sensors 2008 6 Figure 5. PSi RM res
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Sensors 2008 8 2. Pacholski C.; Sar
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