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INDICE SUMMARY pag. i RIASSUNTO Chapter 1:Porous Silicon (PSi) pag. ii Introduction pag. 1 Materials and methods pag. 10 Thue-Morse: aperiodic multilayers made of PSi pag. 16 Optical sensing of chemical compounds using porous silicon devices Chapter 2:Chemical and Biological functionalization of PSi surface pag. 22 Introduction: PSi surface chemical functionalization pag. 29 DNA Optical Detection Based on PSi Technology pag. 33 Optical biosensors for the detection of analytes of clinical and industrial interest. pag. 40 Optical evidence of protein structural changes in PSi devices pag. 55 Oligonucleotides direct synthesis on PSi chip pag. 61 PSi-based optical biosensors and biochips Chapter 3:Hybrid systems based on PSi and biocompatible materials pag. 63 Introduction: PSi hybrid systems pag. 67 PSi-polymer composite matrix for an innovative class of optical biosensors pag. 70 A polymer modified PSi optical device for biochemical sensing pag. 73 Bio/NON Bio Interfaces for a new class of proteins Microarrays Chapter 4: …towards the Lab-on-Chip pag. 77 Introduction to Lab-on-chip technologies pag. 82 Microsystems Based on PSi-Glass Anodic Bonding technologies pag. 87 Laser oxidation micropatterning of a PSi based biosensor for multianalytes microarrays Appendix pag. 95
Page 1: BIOCHIP AND LAB-ON-CHIP FOR GENOMIC
Page 5: The most beautiful thing we can exp
Page 9 and 10: RIASSUNTO Una delle più importanti
Page 11 and 12: molecolare, la chimica delle superf
Page 13 and 14: dell’ordine dei 10-100 nm. Questo
Page 15 and 16: Un caso particolarmente interessant
Page 17 and 18: concentrazione aumenta la bagnabili
Page 19 and 20: Reflectivity (a.u.) 1.4 1.2 1.0 0.8
Page 21 and 22: Di fondamentale importanza è la sc
Page 23: Chapter 1 Porous Silicon
Page 26 and 27: Figure 1: schematic of a biosensor
Page 28 and 29: In the past two decades, the biolog
Page 30 and 31: A simplified mechanism for the chem
Page 32 and 33: After the etching, the next step to
Page 34 and 35: Materials and Methods: Porous Silic
Page 36 and 37: The morphology of the porous silico
Page 38 and 39: Reflectivity (u.a) 1.0 0.8 0.6 0.4
Page 40 and 41: Thue-Morse: aperiodic multilayers m
Page 42 and 43: phys. stat. sol. (c) 4, No. 6 (2007
Page 44 and 45: phys. stat. sol. (c) 4, No. 6 (2007
Page 46 and 47: Optical sensing of chemical compoun
Page 48 and 49: line which slope is the optical pat
Page 50 and 51: 1942 L. De Stefano et al.: Quantita
Page 52 and 53: n 1944 L. De Stefano et al.: Quanti
Page 55: Chapter 2 Chemical and Biological f
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Thermal hydrosilylation provides a
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10. Robins EG, Stewart MP, Buriak J
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Sensors 2007, 7, 214-221 Full Resea
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Sensors 2007, 6 Table 1. PSi layers
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Sensors 2007, 6 The characteristic
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Sensors 2007, 6 The sensor response
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Examples of optical biosensors for
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esearch articles De Stefano et al.
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esearch articles De Stefano et al.
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INSTITUTE OF PHYSICS PUBLISHING JOU
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Nanostructured biosensors S2021 in
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Nanostructured biosensors S2023 A B
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Nanostructured biosensors S2025 Fig
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Nanostructured biosensors S2027 5.
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Optical evidence of protein structu
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ellipsometry (Horiba-Jobin-Yvon, mo
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concentration variations of 0.022 (
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Figure 6: (a) Simulated reflectivit
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Oligonucleotides direct synthesis o
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Porous silicon-based optical biosen
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In this work, we review our recent
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In Fig. 2, the red shifts of the m
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Chapter 3 Hybrid systems based on P
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Schematic of a PSi device as etched
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Porous silicon-polymer composite ma
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Introduction Due to their widesprea
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Organic compound Refractive index I
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Bio/NON Bio Interfaces for a new cl
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5 10 15 20 25 30 35 40 45 50 55 Pro
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5 10 15 20 25 30 35 aspecific bindi
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Chapter 4 …towards the Lab-on-Chi
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Figure 1: (1) An example of bulk mi
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Figure 4: Different types of bondin
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30. Camon H and Moktadir Z 1997 Sim
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Sensors 2006, 6, 680-687 Full Resea
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Sensors 2006, 6 682 From the optica
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Sensors 2006, 6 684 Intensity (a.u.
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Sensors 2006, 6 686 chamber and on
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Laser oxidation micropatterning of
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383 single components of a complex
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Fig. 2: functionalization steps of
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387 [3] R. Herino, G. Bomchil, K. B
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J20. L. De Stefano, A. Vitale, I. R
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C2. Luca De Stefano, F. G. Della Co
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C35. L. Rotiroti, E. De Tommasi, I.
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INSTITUTE OF PHYSICS PUBLISHING JOU
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LDeStefanoet al Reflectance 40 35 3
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LDeStefanoet al [2] De Stefano L, M
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J. Phys.: Condens. Matter 19 (2007)
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Extremophiles DOI 10.1007/s00792-00
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Extremophiles setup, used to measur
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Extremophiles ∆λ (nm) 1.4 1.2 1.
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Sensors 2008 2 analytes present in
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Sensors 2008 4 Figure 2. Schematic
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Sensors 2008 6 Figure 5. PSi RM res
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Sensors 2008 8 2. Pacholski C.; Sar
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