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CHAPTER XV: TECHNICAL SPECIFICATIONS & OTHER IMPORTANT REQUIREMENTS ITEM NO.1 – ISOLATION OF MUTATIONS IN RICE GENES USING TILLING (TECHNICAL SERVICE) [FILE REF: 34/CCMB/APP/417/P12) SPECIFICATIONS FOR ISOLATION OF MUTATIONS IN RICE GENES USING TILLING (TECHNICAL SERVICE) Purpose: Use TILLING to isolate mutations in rice genes. Specifications: 1. 8-10 mutations are to be isolated/gene. 2. A total of 12 rice genes that are indicated by CCMB are to be used in this analysis. 3. The mutant population of 10,000 M2 families (~10-15 plants/family) of rice plants shall be provided by CCMB. It is to be grown and maintained in India only. 4. TILLING is to be done in India only. 5. Details of the methodology that is to be used in TILLING using this population are to be provided including the scheme for pooling and gridding. 6. The time frame for isolation of mutations is to be provided. Essential Qualifications: Established expertise in growing large mutagenized populations of plants, isolation of DNA from plants on a large scale, pooling the DNA in grids and using TILLING for allele discovery in crop plants with evidence of the same. IMPORTANT: Bids must be submitted in TWO PARTS (TECHNICAL & PRICE BIDS) Some of the provisions of this tender document which are applicable in case of supply, installation, and commissioning of equipments MAY NOT apply in the case of providing of Technical Services. All such information may be marked as Not Applicable /NA in your bid papers. Price details in the Price Bid are to be submitted as explained below. If the price bid is not submitted in this format, the quotation shall be treated as unresponsive and rejected summarily. (PRICES FOR BOTH THE OPTIONS GIVEN BELOW MUST BE QUOTED IN THE PRICE BID PART) Option1: Price for the service has to be quoted by inclusive of all services and materials like reagents, including for DNA isolation, are procured by the service provider. Option2: Price for the service if CCMB procures all of the required reagents for DNA isolation and supplies them to service provider. In this option, bidder has to attach a list of all of the required reagents / chemicals etc. for DNA isolation and supplies them to service provider. The list of reagents, source of reagents (including catalogue numbers) and quantity of reagents that are required needs to be included in the quote. BID DOCUMENT (YC) Page 28 of 63
ITEM NO.2 - FLUORESCENCE MICROSCOPE, QTY: 1 NO. (FILE REF: 34/CCMB/APP/387/P12) SPECIFICATIONS FOR HIGH END RESEARCH GRADE UPRIGHT FLUORESCENCE MICROSCOPE: Binocular Upright Microscope with 12v 100w halogen lamp, 10x eyepieces with more field of view, objectives semi apochromat 10x, 20x, Plan apochromat 40x, 63x, 100x with DIC attachment for above objectives, condenser for bright field, dark field, phase, DIC applications, Objective nose piece with 6 positions, Neutral density filters for both bright field and Fluorescence, Blue and green filters for transmission light path, 100w mercury lamp with automatic mercury lamp alignment feature. Fluorescence filters for DAPI, FITC, CY3, CY5, separate narrowband GFP filter, Fully apochromatic corrected optical path for fluorescence, pixel shift should not be present while acquiring DIC & fluorescence imaging. X&Y stage with right hand control, camera port, high end fire wire digital colour camera with 1.3 mega pixel resolution, pixel size approximately 6 microns, good full well capacity and dynamic range, peltier based cooled camera. High end software for image acquisition with multichannel imaging, measurements, fast live imaging mode, high resolution acquisition mode. BID DOCUMENT (YC) Page 29 of 63
Page 1 and 2: CENTRE FOR CELLULAR & MOLECULAR BIO
Page 3 and 4: VERY IMPORTANT: Pre-Bid Conference
Page 5 and 6: CHAPTER - II: INSTRUCTION TO BIDDER
Page 7 and 8: 10 Documents Establishing Bidder’
Page 9 and 10: E. OPENING AND EVALUATION OF BIDS T
Page 11 and 12: CHAPTER - III: GENERAL CONDITIONS O
Page 13 and 14: 15.2This warranty shall remain vali
Page 15 and 16: CHAPTER - IV: SPECIAL CONDITIONS OF
Page 17 and 18: 17 Supplier Integrity 17.1The Suppl
Page 19 and 20: CHAPTER - VI: BID SECURITY FORM Whe
Page 21 and 22: CHAPTER - VIII: BIDDER’S PERFORMA
Page 23 and 24: CHAPTER - X: DEVIATION STATEMENT FO
Page 25 and 26: CHAPTER - XII: CONTRACT FORM THIS A
Page 27: CHAPTER XIV: QUALIFICATION REQUIREM
Page 31 and 32: 3. Ambient temperature: 42 deg. C 4
Page 33 and 34: ITEM NO.5 - HIGH RESOLUTION FTIR SY
Page 35 and 36: ITEM NO.6 - PROTEIN PURIFICATION SY
Page 37 and 38: ITEM NO.7 - PHOSPHOR IMAGER ALONG W
Page 39 and 40: ITEM NO.8 - UPRIGHT FLUORESCENCE MI
Page 41 and 42: ITEM NO.9 - ADVANCED MOTORIZED INVE
Page 43 and 44: ITEM NO.10 - SPECTRAL CONFOCAL MICR
Page 45 and 46: ITEM NO.11 - HIGH RESOLUTION MASS S
Page 47 and 48: ITEM NO.12 - HIGH SENSITIVITY CD SP
Page 49 and 50: 2. All the above mentioned technica
Page 51 and 52: . The computer and printer offered
Page 53 and 54: 2. Cost should include three years
Page 55 and 56: 2. Capacity : 8 x 5 ml or more Maxi
Page 57 and 58: ITEM NO.17 - ZEBRA FISH IMAGING AND
Page 59 and 60: ITEM NO.18 - HIGH CONTENT SCREENING
Page 61 and 62: 7. Software supplied should be the
Page 63: Main system including subsystems, c

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