pdf tender document information - CCMB
pdf tender document information - CCMB
pdf tender document information - CCMB
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ITEM NO.6 - PROTEIN PURIFICATION SYSTEM, QTY: 4 NOS. (FILE REF:<br />
34/<strong>CCMB</strong>/APP/403/P12)<br />
SPECIFICATIONS FOR PROTEIN PURIFICATION SYSTEM<br />
1. Gradient : Binary Gradient with multiple buffer selection facility.<br />
2. Flow range : 0.01 to 10.00 ml/min<br />
3. Pressure range : 0- 3500 psi with programmable upper and lower limits<br />
4. Mixing : High pressure mixing<br />
5. Sample injection : Auto injector / Manual injector<br />
6. Sample loops : Various sizes covering 100 ul – 5ml<br />
7. Detection : Variable wave length UV/Vis detector, PH, Conductivity<br />
8. Wavelength range : 190 – 700 nm<br />
9. Conductivity range : 0-500 ms/cm<br />
10. PH Range : 2-12<br />
11. Fraction collector : Time, Drop and Peak fractionation modes.<br />
12. System control : PC-based along with software for control and data acquisition<br />
13. Cold room compatibility : Entire system along with computer should be cold room compatible.<br />
14. Biocompatibility : System should be Biocompatible.<br />
15. Column Cross compatibility : Required (All adopters and accessories to make the system<br />
Compatible for third party columns to be included in the offer)<br />
16.Column and Media : Please quote separately (including the column list enclosed)<br />
17. Power : All components including subsystems, accessories and Computers<br />
must be compatible with 220 volts, 50Hz electrical power<br />
List of Columns required by <strong>CCMB</strong><br />
A. Preparative-grade or semi-prep Columns for gel-filtration or size exclusion chromatography:<br />
Columns with bed volume of approx. 100-150 ml and separation ranges of 1 kDa-100 kDa and 10 kDa-500 kDa<br />
(other separation range should also be provided if available)<br />
(Columns of different resins (bead size is different and thus resolution) should be included)<br />
B. Analytical-grade Gel-filtration Columns:<br />
Columns with bed volume of approx. 20-30 ml and separation ranges of 1 kDa-100 kDa and 10 kDa-500 kDa each<br />
(Columns of different resins (bead size is different) should be included)<br />
C. Cation-Exchange FPLC Columns:<br />
1. Strong-binding resin with bed volume of approx. 20 ml<br />
2. Weak-binding resin with bed volume of approx. 20 ml<br />
D. Anion-Exchange FPLC Columns:<br />
1. Strong-binding resin with bed volume of approx. 20 ml<br />
2. Weak-binding resin with bed volume of approx. 20 ml<br />
BID DOCUMENT (YC)<br />
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