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pdf tender document information - CCMB

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ITEM NO.6 - PROTEIN PURIFICATION SYSTEM, QTY: 4 NOS. (FILE REF:<br />

34/<strong>CCMB</strong>/APP/403/P12)<br />

SPECIFICATIONS FOR PROTEIN PURIFICATION SYSTEM<br />

1. Gradient : Binary Gradient with multiple buffer selection facility.<br />

2. Flow range : 0.01 to 10.00 ml/min<br />

3. Pressure range : 0- 3500 psi with programmable upper and lower limits<br />

4. Mixing : High pressure mixing<br />

5. Sample injection : Auto injector / Manual injector<br />

6. Sample loops : Various sizes covering 100 ul – 5ml<br />

7. Detection : Variable wave length UV/Vis detector, PH, Conductivity<br />

8. Wavelength range : 190 – 700 nm<br />

9. Conductivity range : 0-500 ms/cm<br />

10. PH Range : 2-12<br />

11. Fraction collector : Time, Drop and Peak fractionation modes.<br />

12. System control : PC-based along with software for control and data acquisition<br />

13. Cold room compatibility : Entire system along with computer should be cold room compatible.<br />

14. Biocompatibility : System should be Biocompatible.<br />

15. Column Cross compatibility : Required (All adopters and accessories to make the system<br />

Compatible for third party columns to be included in the offer)<br />

16.Column and Media : Please quote separately (including the column list enclosed)<br />

17. Power : All components including subsystems, accessories and Computers<br />

must be compatible with 220 volts, 50Hz electrical power<br />

List of Columns required by <strong>CCMB</strong><br />

A. Preparative-grade or semi-prep Columns for gel-filtration or size exclusion chromatography:<br />

Columns with bed volume of approx. 100-150 ml and separation ranges of 1 kDa-100 kDa and 10 kDa-500 kDa<br />

(other separation range should also be provided if available)<br />

(Columns of different resins (bead size is different and thus resolution) should be included)<br />

B. Analytical-grade Gel-filtration Columns:<br />

Columns with bed volume of approx. 20-30 ml and separation ranges of 1 kDa-100 kDa and 10 kDa-500 kDa each<br />

(Columns of different resins (bead size is different) should be included)<br />

C. Cation-Exchange FPLC Columns:<br />

1. Strong-binding resin with bed volume of approx. 20 ml<br />

2. Weak-binding resin with bed volume of approx. 20 ml<br />

D. Anion-Exchange FPLC Columns:<br />

1. Strong-binding resin with bed volume of approx. 20 ml<br />

2. Weak-binding resin with bed volume of approx. 20 ml<br />

BID DOCUMENT (YC)<br />

Page 35 of 63

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