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ITEM NO.13 – HIGH THROUGHPUT REAL TIME PCR SYSTEMS, QTY: 2 NOS. + 3 NOS. (FILE
REF: 34/CCMB/APP/410/P12)
SPECIFICATIONS OF HIGH THROUGHPUT REAL TIME PCR SYSTEMS
I) High throughput Real time PCR Systems --- 2 nos
Applications: a) Gene Expression studies
b) Absolute quantitation and relative quantitation
c) Allelic discrimination
d) Genotyping
e) HRM analysis
Specifications:
Block Format 96 well / 384 well interchangeable.
Fluorescence excitation and 450 nm to 650nm
Emission range
500 nm to 700 nm
Thermal cycling system Peltier based
Temperature range 4 ºC to 100 ºC
Average Ramping rates 3.0 C/sec or better
Temperature accuracy ± 0.3 ºC or better
Temperature uniformity ± 0.5 ºC or better
Sensitivity Detection down to one copy
Reaction volume 3 to 20 microlitres for 384 well plates
Standard features required:
II)
User interchangeable blocks 384 well to 96 well blocks with no calibrations requirements for every block change.
Multiplexing capability upto five colours or more.
Software for all the above mentioned applications, primers & probes design software etc with multi user license to
be included with the system.
Provision to add new filters/ dyes.
Provision to run with or without passive reference dye.
The system should be compatible for use with third party consumables and kits.
Should be able to store at least 100 or more data files.
Maximum run time should not be more than 80 to 90 minutes with standard kits and not more than 40 minutes
with fast reagents and consumables.
Should be able to upload/download protocols, data files etc using an USB interface.
Provision for sensitized emission based measurements for at least four/ five colours with independently selectable
emission and excitation filters.
High throughput Real time PCR Systems for all the above applications --- 3 nos
with all the specifications, standard features as given in item I and in
addition having the following features
System offered should be upgradable to high density array block at any time.
Should be FDI approved and validated for diagnostic applications.
III)
To offer Optionally (for items I & II)
Kits and reagents for Real Time PCR, SNP Genotyping.
Reagents and consumables for fast runs
96 well block.
High density array block.
384 well plates compatible with the above systems.
Required kits for diagnostic applications for item (II).
Important clauses:
a. Main system including subsystems, computers and accessories should operate on 220V, 50 Hz mains power.
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